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首页 > 过刊浏览>2020年第21卷第2期 >359-368. DOI:10.13430/j.cnki.jpgr.20190627002
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环境变异对青稞热稳定蛋白含量及蛋白质Z的影响
作者:
作者单位:

青海大学农林科学院／青海省青稞遗传育种重点实验室／国家麦类改良中心青海青稞分中心

基金项目:

国家自然科学基金（31660388）；国家大麦（青稞）产业技术体系（CARS-05）；青海省科技厅应用基础研究项目（2019-ZJ-7075）；青海省中央引导地方专项；省部共建青稞和牦牛种质资源与遗传改良国家重点实验室开放课题（XZNKY-2019-C-007K05）


The Effect of Environmental Variation on the Content of Heat-stabilized Protein and Protein Z in Hulless Barley
Author:
Affiliation:

Academy of Agricultural and Forestry Sciences, Qinghai University/Qinghai Key Laboratory of Hulless Barley Genetics and Breeding/ Qinghai Subcenter of National Hulless Barley Improvement, Qinghai Xining

Fund Project:

Natural Science Foundation of China (31660388), China Agriculture Research System (CARS-05), the Project of Application and Fundamental Research in Qinghai Science and Technology Department ( 2019-ZJ-7075), the Project of Central Government Directing local Governments in Qinghai; Open topic of State Key Laboratory of Barly and Yak Germplasm Resourse and Genetic Improvement (XZNKY-2019-C-007K05)

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    摘要:

    热稳定蛋白是衡量麦芽品质的重要指标，为探明青稞籽粒和麦芽热稳定蛋白的含量、蛋白质 Z 的组成特征以及影响条件。本文以 3 份青稞和 1 份对照大麦品种 Gairdner 为试验材料，对青稞籽粒及其麦芽的热稳定蛋白进行分析与鉴定，研究了不同生态环境下青稞热稳定蛋白含量和蛋白质 Z 的组成特征，同时筛选出了优异啤用品质青稞品种（品系）。结果表明，青稞发芽温度为 20℃，发芽时间为 72h，培养溶液 PH 为 5 时，发芽及焙焦条件下最有利于青稞热稳定蛋白总含量及蛋白质Z 的累积。利用该发芽条件筛选种植于西宁、湟源和海晏的青稞资源，发现种植于西宁的青稞种子和发芽后热稳定蛋白质总含量最低，但是焙焦后热稳定蛋白质和蛋白质 Z 含量最高；同时从 150 份青稞资源中筛选出热稳定蛋白含量及蛋白质 Z 条带清晰、含量高的优异资源 15 份。本研究结果为酿造青稞品种选育、啤用青稞和麦芽质量评价指标提供理论依据。

    Abstract:

    Heat-stabilized protein is an important index to measure the quality of malt. In order to explore the content of heat-stabilized protein, the composition characteristics of protein Z and the influencing conditions in barley grains and malt, three hulless barleys (Liulengzi changmang baiqingke, Qingpi qingke and 9820) and one hulled barley (Gairdner ) were used to analyze and identify the heat-stabilized protein of hulless barley grains and malt. Then the content of the heat-stabilized protein of hulless barley and the composition characteristics of protein Z were studied in different ecological environments. The barley lines with excellent brewing quality were selected. The results showed that the optimal barley germination conditions (20℃, 72h and PH 5) enabled the accumulation of heat-stabilized protein and protein Z in germination and kinling. The hulless barleys, which were planted and harvested at three locations (Xining, Huangyuan and Haiyan) with different climatic conditions, were tested for the content of grain. A lowest bud heat-stabilized protein, but the highest heat-stabilized protein and protein Z after kinling, were observed from the grains in Xining. Meanwhile, fifteen excellent resources with high content of heat-stable protein were identified by testing 150 hulless barley germplasm resources. Thus, the results gained from this study will provide theoretical basis in breeding for hulless malting barley varieties with the qualified indexes.

    参考文献
    [1] 郭本兆.青海经济植物志[M]. 西宁:青海人民出版社, 1987:701.Guo B Z. Qinghai Economic Flora[M]. Xining: Qinghai People''s Publishing House, 1987: 701.
    [2] 姚晓华,吴昆仑. 青稞脂质转运蛋白基因 blt4.9 的克隆及其对非生物胁迫的响应[J]. 作物学报, 2016, 42(3): 399-406.Yao X H, Wu K L. Isolation of blt4.9 Gene Rncoding LTP Protein in Hulless Barley and its Response to Abiotic Stresses[J]. Acta Agronomica Sinica, 2016, 42(3): 399-406.
    [3] 陈霞, 石维忱, 赵淑娟, 姜晓雷, 李明达, 赵睿, 赵长新. 不同品种大麦热稳定蛋白—蛋白质Z与LTP蛋白耐热温度的研究[J]. 食品与发酵工业, 2010, 36(5):32-35.Chen X , Shi W C, Zhao S J, Jiang X L, Li M D, Zhao R, Zhao C X. Heat Resistance of Heat-stable Protei-Protein Z and LTP in Different Barley[J]. Food and Fermentation Industries,2010, 36(5):32-35.
    [4] Dale C J, Young T W, Brewer S. Amino acid analysis of beer polypeptides[J]. Journal of the Institute of Brewing,2013, 95(2):89-97.
    [5] Hollemans M, Tonies A. The role of specific proteins in beer foam[J]. Proc Congr Eur Brew,1989, 22: 561-568.
    [6] Bobálová J, Petry-Podgórska I, La?tovi?ková M, Chmelík J. Monitoring of malting process by characterization of glycation of barley protein Z[J]. European Food Research and Technology, 2010, 230(4):665-673.
    [7] Evans D E, Hejgaard J. The Impact of Malt Derived Proteins on Beer Foam Quality. Part I. The Effect of Germination and Kilning on the Level of Protein Z4, Protein Z7 and LTP1[J]. Journal of the Institute of Brewing, 2012, 105(3):159-170.
    [8] Evans D E, Sheehan M C, Stewart D C. The Impact of Malt Derived Proteins on Beer Foam Quality. Part II: The Influence of Malt Foam-positive Proteins and Non-starch Polysaccharides on Beer Foam Quality[J]. Journal of the Institute of Brewing, 2012, 105(3):171-178.
    [9] 孙付保, 陈霞, 王晓丹, 程贺, 赵长新. 国产主要东北大麦和华东大麦热稳定蛋白的比较[J]. 中国酿造, 2010, 29(10):81-83.Sun F B, Chen X, Wang X D, Cheng H, Zhao C X. Comparison of Heat-stable Protein in Chinese Northeast and East Barleys[J]. China Brewing, 2010, 29(10):81-83.
    [10] 王凤敏, 粟建光, 龚友才, 戴志刚, 陈基权, 郑海燕, 李燕. 不同红麻种子耐老化性差异及热稳定蛋白的研究[J]. 植物遗传资源学报, 2010(1):5-9.Wang F M, Su J G, Dai Z G, Chen J Q, Zheng H Y, Li Y. Difference of Aging Tolerance and Heat-Stable Proteins in Seeds of Difference Kenaf Cultivars[J]. Journal of Plant Genetic Resources, 2010, 11(1):5-9(5).
    [11] Bradford M M. A Rapid method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem[J]. Analytical Biochemistry, 1976, 72(s 1–2):248–254.
    [12] 吴晓亮, 辛萍萍, 张志娥, 陈晓玲, 陶澜, 卢新雄. 水稻种子室温贮藏最适含水量及其热稳定蛋白的研究[J]. 中国农业科学, 2006, 39(11):2214-2219.Wu X L, Xin P P, Zhang Z E, Chen X L, Tao L, Lu X X. Studies on Optimum Moisture Content and Heat-Stable Protein of Rice Seeds Stored at Room Temperature[J]. Scientia Agricultura Sinica, 2006, 39(11):2214-2219.
    [13] 付深造, 辛霞, 张志娥, 陈晓玲, 辛萍萍, 卢新雄. 利用热稳定蛋白特异条带鉴别籼粳稻的方法研究[J]. 植物遗传资源学报, 2011, 12(1):19-24(6).Fu S Z, Xin X, Zhang Z E, Chen X L, Xin P P, Lu X X. Method for Identifying Indica-Japonica Rice by Using Specific Heat-Stable Protein Bands[J]. Journal of Plant Genetic Resources, 2011, 12(1):19-24(6).
    [14] Lapitan N L V, Hess A, Cooper B, et al. Differentially expressed genes during malting and correlation with malting quality phenotypes in barley (Hordeum vulgare L.) [J]. Theoretical and Applied Genetics, 2009, 118(5):937-952.
    [15] Knorr V, Kerpes R, Wieser H, et al. Production and application of barley malt extract with high peptidase activity for the degradation of gluten in wort[J]. European Food Research and Technology, 2016, 242(4):585-597.
    [16] 潘永东, 包奇军, 张华瑜, 柳小宁. 啤酒大麦新品种在甘肃省中部地区的适应性研究[J]. 大麦与谷类科学, 2008,(3):1-3.Pan Y D, Bao Q J, Zhang H Y, Liu X N. Adaptability of New Barley Varieties in Central Gansu Province[J]. Barley and Cereal Sciences, 2008, (3):1-3.
    [17] Asres T, Tadesse D, Wossen T, Sintayehu A. Performance Evaluation of Malt Barley: from Malting Quality and Breeding Perspective [J]. Journal of Crop Science and Biotechnology, 2018, 21(5):451-457.
    [18] 孙俊, 张天雪, 孙丽华, 徐凯, 赵长新. 大麦发芽过程中热稳定蛋白质的变化及部分生化特性[J]. 大连轻工业学院学报, 2008, 27(3):223-227.Sun J, Zhang T X, Sun L H, Xu K, Zhao C X. Characterization of Heat-Stable Protein in Germinating Barley Seeds[J]. Journal of Dalian Polytechnic University, 2008, 27(3):223-227.
    [19]周芸芸, 陈爽, 王国华, 陆英, 王荣, 王小溪, 王安平, 林智平. 制麦过程中蛋白酶酶活力变化和蛋白质溶解情况的研究[J]. 啤酒科技, 2017, (12): 42-51.Zhou Y Y, Chen S, Wang G H, Chen Y, Wang R, Wang X X, Wang A P, Lin Z P. Changes of Protease Activity and Protein Dissolution in Wheat Making Process[J]. Beer Science and Technology, 2017, (12): 42-51.
    [20] Brandt A, Svendsen I, Hejgaard J. A plant serpin gene. Structure, organization and expression of the gene encoding barley protein Z4. European Journal of Biochemistry, 2010, 194(2):499-505.
    [21] Fasoli E, Aldini G, Regazzoni L, Kravchuk A V, Citterio A, Righetti P G. Les Maitres de l’Orge: proteome content your mug[J]. Journal of Proteome Research, 2010, 9(10): 5262-9.
    [22] Han Y P, Wang J J, Li Y X, Hang Y. Yin X S, Li Q. Circular dichroism and infrared spectroscopic characterization of secondary structure components of protein Z during mashing and boiling processes[J]. Food Chemistry, 2015, 188:201-209.
    [23] 吴晓明, 陈锦新, 吴桂玉.啤酒大麦品种的蛋白质含量和环境变异研究[J]. 大麦科学, 2004, (1):27-29.Wu X M, Chen J X, Wu G Y. Study on Protein Content and Environmental Variation of Malting Barley Cultivar[J]. Barley Science, 2004,(1):27-29.
    [24] 陈锦新, 张国平, 汪军妹, 陈仲华, 周体耀.氮肥运筹对大麦β-葡聚糖酶活性和麦芽品质的影响[J]. 作物学报, 2004, 30(1):47-51.Chen J X, Zhang G P, Wang J M, Chen Z H, Zhou T Y. The Effects of Timing of N Application on Barley β-glucanase Activity and Malt Quality[J]. Acta Agronomica Sinica, 2004, 30(1):47-51.
    [25] 孙立军, 吕耀昌, 孔繁玲, 刘文欣.大麦高蛋白种质遗传稳定性分析[J]. 大麦科学, 2002, (3):8-13.Sun L J, Lv Y C, Kong F L, Liu W X. Genetic Stabilty Analysis of High-protein Germplasms of Barley[J]. Barley Science, 2002, (3):8-13.
    [26] Steiner E, Gastl M, Becker T. Protein changes during malting and brewing with focus on haze and foam formation: a review[J]. European Food Research and Technology, 2011, 232(2):191-204.
    [27] Evans D E, Sheehan M C, Stewart D C. The Impact of Malt Derived Proteins on Beer Foam Quality. Part II: The Influence of Malt Foam-positive Proteins and Non-starch Polysaccharides on Beer Foam Quality[J]. Journal of the Institute of Brewing, 2012, 105(3):171-178.
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姚晓华,王越,白羿雄,等.环境变异对青稞热稳定蛋白含量及蛋白质Z的影响[J].植物遗传资源学报,2020,21(2):359-368.

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  • 收稿日期:2019-06-27
  • 最后修改日期:2019-08-07
  • 录用日期:2019-08-13
  • 在线发布日期: 2020-03-17
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