Abstract:In order to produce the elite germplasm resource for tea breeding, we deployed the artificial hybridization approach to generate the F1 hybrids by crossing six elite tea varieties including Fuding Dabaicha (mother) with another variety Baojing Huangjincha 1, which showed a specific phenotype "golden rhyme" but weak adaptation ability. We found that Baojing Huangjincha 1 pollen exhibited a strong living force whose fresh pollen life force reached to 94.22% by taking researching on Baojing Huangjincha 1 pollen life force, different hybrid combination strong rate, hybrid F1 generation seedling rate, F1 generation excellent single plant selection rate, etc.. Under room temperature, 4℃ and -20℃ conditions, its pollen was able to survive up to 7d, 30d and 40d, respectively. The seed setting rates of six hybrid combinations were variable from 15.25% (Bixiangzao) to 31.32% (Fuding Dabaicha) with the average of 21.88%, while the seedling rates were arranged from 50.77% (Bxiangzao) to 66.65% (Tieguanyin) with a mean value of 59.62%. No significant difference was observed in seed setting rate and seedling rate among different treatments (combinations and years). By taking use of three rounds of selecting excellent strains and identifying asexual reproduction ability, a total of 60 strains were finally accepted with a selection rate of 2.35%. The rate of elite hybrids was much higher than that of naturally-occurring hybridization offspring (0.04%). Furthermore, we conducted a comprehensive evaluation of biochemical composition, yield and quality related traits in selected hybrids. Three new strains, which were subjected for the regional test, showed the growth potential and excellent sensory quality (especially with aroma prominent), much better than the father Baojing Huangjincha 1.