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首页 > 过刊浏览>2021年第22卷第1期 >95-101. DOI:10.13430/j.cnki.jpgr.20200409002
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小麦耐热分子标记Xwmc44的鉴定及分析
作者:
作者单位:

1 河北省农林科学院遗传生理研究所 / 河北省植物转基因中心重点实验室,石家庄 050051; 2南京农业大学植物保护学院,南京 210095; 3 石家庄市农林科学研究院,石家庄 050000

基金项目:

河北省现代农业创新工程（ 494-0402-YBN-S2XB， 494-0402-YBN-CTGQ）；河北省重点研发计划项目（ 20326309D）


Identification and Analysis of Heat-tolerant Molecular Marker Xwmc44 in Wheat
Author:
Affiliation:

1 Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/ Key Laboratory of Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051; 2 College of Plant Protection, Nanjing Agricultural University, Nanjing 210095; 3 Shijiazhuang Academy of Agricultural and Forestry Sciences, Shijiazhuang 050000

Fund Project:

Technological Innovation Project of Modern Agriculture of Hebei Province（ 494-0402-YBN-S2XB， 494-0402-YBN-CTGQ）， The Key Research and Development Program of Hebei Province（ 20326309D）

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    摘要:

    耐热育种是我国小麦育种的一个重要目标。寻找与目标性状关联的分子标记，可克服常规育种的盲目性，提高分 子标记辅助选择育种的准确性。本研究对 169 份小麦品种（系）的千粒重、籽粒长度、籽粒宽度、籽粒表面积和籽粒周长 5 个 籽粒相关性状的耐热指数进行鉴定，通过简单关联分析获得耐热相关分子标记 Xwmc44。聚丙烯酰胺凝胶电泳分析表明： Xwmc44 具有 I、 II、 III 3 个等位变异类型。 I 型等位变异与千粒重、籽粒宽度、籽粒表面积和籽粒周长 4 个籽粒性状的热感指 数显著正相关（ P<0.05）， I 型等位变异小麦品种（系）的热感指数平均值大于 1； II 型等位变异与千粒重热感指数极显著负相 关（ P<0.01），与籽粒宽度和籽粒周长 2 个籽粒性状的热感指数显著负相关（ P<0.05）， II 型小麦品种（系）的耐热指数平均值 小于 1，为优良等位变异； III 型等位变异与小麦籽粒性状热感指数都不相关。研究结果表明： Xwmc44 与小麦耐热性密切相 关，其中 II 型等位变异是耐热优良等位变异。这为小麦耐热分子育种提供了参考信息。

    Abstract:

    High temperature at reproductive stage destabilizes grain yield and quality especially in warm climate wheat production region. Breeding for heat-tolerant wheat varieties is an important target of wheat breeding in China， while identification of the molecular markers associated with the heat tolerant traits can accelerate this process. The heat-tolerant indexes of five kernel traits including thousand kernel weight， kernel length and kernel width， kernel surface area and kernel circumference were investigated by taking use of 169 wheat varieties（ lines）， and genotyping with SSR marker Xwmc44 identified three allelic variants I， II and III. A significant positive correlation between variant I and heat-tolerant indexes of four kernel traits（ thousand kernel weight， kernel width， kernel surface area and kernel circumference） was observed. The average values of heat-tolerant indexes of kernel traits in wheat varieties（ lines） with I allelic variation was greater than 1. The wheat varieties with variant II was negatively correlated with the heat tolerance index of thousand kernel weight （ P<0.01）， and negatively correlated with the heat-tolerance index of two kernel traits including kernel width and kernel circumference（ P<0.05） . The average value of heat-tolerance indexes of kernel traits in wheat varieties （ lines） with II allelic variation was less than 1. No detectable association was observed in variant III with the heat-tolerance indexes. These results showed that Xwmc44 was related to heat tolerance of wheat， and II allelic variation was a superior allele for heat tolerance of wheat. This will provide more information for heat-tolerant wheat breeding with molecular markers.

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刘子会,刘畅,彭义峰,等.小麦耐热分子标记Xwmc44的鉴定及分析[J].植物遗传资源学报,2021,22(1):95-101.

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  • 收稿日期:2020-04-09
  • 最后修改日期:2020-04-27
  • 录用日期:2020-05-18
  • 在线发布日期: 2021-01-07
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