2025年6月13日 10:29 星期五
首页 > 过刊浏览>2021年第22卷第2期 >399-406. DOI:10.13430/j.cnki.jpgr.20200827001
PDF HTML阅读 XML下载 导出引用 引用提醒
利用KASP标记筛选含rhg1和Rhg4位点的大豆抗病资源
作者:
作者单位:

河南省作物分子育种研究院/国家大豆改良中心郑州分中心/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室/河南省农业科学院经济作物研究所,郑州,450002

基金项目:

国家重点研发项目(2017YFD0101400); 河南省科技攻关项目(202102110158)共同资助


Marker-Assisted Screening of Soybean Cyst Nematode Germplasms Harboring Resistance Loci rhg1 and Rhg4
Author:
  • LIAN Yun

    LIAN Yun

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Hai-chao

    LI Hai-chao

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LI Jin-ying

    LI Jin-ying

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHOU Yang

    ZHOU Yang

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • WANG Shi-wei

    WANG Shi-wei

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHANG Hui

    ZHANG Hui

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LEI Chen-fang

    LEI Chen-fang

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • WU Yong-kang

    WU Yong-kang

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHANG Jin-peng

    ZHANG Jin-peng

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • WANG Jin-she

    WANG Jin-she

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LU Wei-guo

    LU Wei-guo

    Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002
    在期刊界中查找
    在百度中查找
    在本站中查找
Affiliation:

Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry?of?Agriculture?and?Rural?Affairs / Henan Provincial Key Laboratory for Oil Crops Inprovement/ Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002

Fund Project:

National Key Research and Development Program of China (2017YFD0101400); Henan Provincial Department of Science and Technology Research Project (202102110158)

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [55]
    • | | | |
  • 文章评论
    摘要:

    大豆胞囊线虫（SCN，Soybean cyst nematode）病是一种世界性大豆病害，培育抗SCN大豆品种是防治SCN的重要措施。本研究利用来自抗SCN主效位点rhg1和Rhg4的2个KASP 标记，对487份大豆材料进行筛选，选择含有抗性位点且农艺性状优异的材料；通过室内接种大豆胞囊线虫2号、4号、5号生理小种和新小种X12，进行抗性鉴定验证其抗性水平，为培育抗病品种提供抗源材料。标记筛选结果表明20份材料含有rhg1和Rhg4这2个主效抗性位点，其中2份材料仅含有Rhg4位点。表型抗性鉴定结果表明，在接种的22份材料中，有1份材料对3个小种表现中抗，5份材料对2个小种表现抗或中抗。其中，1份材料对2号小种表现抗病、4份表现中抗；2份材料对4号小种表现中抗；4份材料对5号小种表现抗病、14份表现中抗；22份材料对新小种X12均表现出感病或中感。因此，本研究从487份材料中筛选出20份含有2个SCN抗性位点并具优异农艺性状的材料，可通过rhg1和Rhg4 位点的累加培育抗病品种。

    Abstract:

    Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is one of the most yield-limiting diseases of soybean worldwide. Releasing SCN-resistant cultivars are the primary basis for controlling SCN. Identification of parent lines showing major resistant loci and desirable agronomic characteristics are prerequisite for breeding resistant cultivars. In this study, a total of 487 soybean accessions were genotyped using two KASP markers that targeted to two resistance loci rhg1 and Rhg4. The accessions, which were identified harboring resistant alleles, were further subjected for resistance inoculated with race-2, race-4, race-5 and race-X12 in the greenhouse. Twenty accessions were characterized simultaneously harboring rhg1 and Rhg4 loci, while two accessions was found with the Rhg4 locus. Testing for SCN resistance suggested that one accession showed moderate resistant to three races and five accessions showed resistant or moderately resistant to two races. One accession was resistant and four accessions were moderately resistant to race-2. Two accessions showed moderately resistant to race-4. Four accessions showed resistant and fourteen moderately resistant to race-5. However, these accessions were turned out to be moderately susceptible or susceptible against race-X12. Collectively, by taking use of 487 soybean accessions this study identified 20 accessions that harbored two major resistant loci and showed desirable agronomic characteristics, might serve as elite parental lines in breeding for resistant cultivars via pyramiding rhg1 and Rhg4.

    参考文献
    [1] Koenning S R, Wrather J A. Suppression of soybean yield potential in the continental United States from plant diseases estimated from 2006 to 2009. Plant Health Progress. 2010.
    [2] Allen T W. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Progress. 2017.
    [3] Niblack T L, Colgrove A L, Colgrove K, Bond J P. Shift in virulence of soybean cyst nematode is associated with use of resistance from PI 88788. Plant Health Progress, 2008, 9: 29.
    [4] Lu H, Tallman J, Hu X, Anderson E, Chamberlin M, Lu G. An innovative method for counting females of Soybean Cyst Nematode with fluorescence imaging technology. Journal of Nematology, 2005, 37: 495–499.
    [5] Brown S, Yeckel G, Heizn R, Clark K, Sleper D, Mitchum M G. A high-throughput automated technique for counting females of Heterodera glycines using a fluorescence-based imaging system. Journal of Nematology, 2010, 42: 201-206.
    [6] 王金社, 卢为国, 李金英, 练云, 魏荷, 李海朝, 雷晨芳. 植物病虫害表型数据采集系统: 中国, 2014SR060158, 2014-5-14.
    Wang J S, Lu W G, Li J Y, Lian Y, Wei H, Li H C, Lei C F. The data acquisition system on the phenotype of plant diseases and insect pests: China, 2014SR060158, 2014-5-14 (in Chinese)
    [7] 练云, 魏荷, 王金社, 雷晨芳, 李海朝, 武永康, 卢为国. 影响大豆胞囊线虫生理小种鉴定因素探讨. 分子植物育种, 2015, 13(6):1259-1264.
    Lian Y, Wei H, Wang J S, Lei C F, Li H C, Wu Y K, Lu W G. A Study on the factors that influence the race-identification of soybean cyst nematode. Molecular Plant Breeding, 2015,13(6): 1259-1264. (in Chinese)
    [8] Grabau Z J, Chen S. Efficacy of organic soil amendments for management of Heterodera glycines in greenhouse experiments. Journal of Nematology, 2014, 46: 267-274.
    [9] Mitchum M G. Soybean resistance to the soybean cyst nematode Heterodera glycines: An update. Phytopathology, 2016, 106: 1444-1450.
    [10] Lian Y, Guo J Q, Li H C, Wu Y K, Wei H, Wang J S, Li J Y, Lu W G. A new race (X12) of soybean cyst nematode in China. Journal of Nematology, 2017, 49: 321-326.
    [12] Cook D E, Lee T G, Guo X, Melito S, Wang K, Bayless A M, Wang J, Hughes T J, Willis D K, Clemente T E, Diers B W, Jiang J, Hudson M E, Bent A F. Copy number variation of multiple genes at Rhg1 mediates nematode resistance in soybean. Science, 2012, 338: 1206-1209.
    [13] Liu S M, Kandoth P K, Warren S D, Yeckel G, Heinz R, Alden J, Yang C, Jamai A, EI-Mellouki T, Juvale P S, Hill J, Baum T J, Cianzio S, Whitham S A, Korkin D, Mitchum M G, Meksem K. A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens. Nature, 2012, 492: 256-260.
    [14] Lee T G, Kumar I, Diers B W, Hudson M E. Evolution and selection of Rhg1, a copy-number variant nematode-resistance locus. Molecular Ecology, 2015, 24: 1774-1791.
    [15] Neupane S, Purintun J M, Mathew F M, Varenhorst A J, Nepal Madhav P. Molecular basis of soybean resistance to soybean aphids and soybean cyst nematodes, Plants (Basel), 2019, 8: 374.
    [16] Concibido V C, Diers B W, Arelli P R. A decade of QTLmapping for cyst nematode resistance in soybean. Crop Science, 2004, 44: 1121-1131.
    [17] Afzal A J, Natarajan A, Saini N, Iqbal M J, Geisler M, Ei Shemy H A, Mungur R, Willmitzer L, Lightfoot D A. The nematode resistance allele at the rhg1 locus alters the proteome and primary metabolism of soybean roots. Plant Physiology, 2009, 151: 1264-1280．
    [18] Kim M, Hyten D L, Bent A F, Diers B W. Fine mapping of the SCN resistance locus rhg1-b from PI 88788. The Plant Genome 2010, 3: 81-89.
    [19] Concibido V C, Lange D A, Denny R L. Genome mapping of soybean cyst nematode resistance genes in ‘Peking’, PI 90763 and PI 88788 using DNA markers. Crop Science, 1997, 37: 258-264．
    [20] Liu S, Kandoth P K, Lakhssassi N, Kang J, Colantonio V, Heinz R, Yeckel G, Zhou Z, Bekal S, Dapprich J, Rotter B, Cianzio S, Mitchum M G, Meksem K. The soybean GmSNAP18 gene underlies two types of resistance to soybean cyst nematode. Nature Communications, 2017, 8: 14822.
    [21] Kandoth P K, Liu S, Prenger E, Ludwig A, Lakhssassi N, Heinz R, Zhou Z, Howland A, Gunther J, Eedson S, Dhroso A, Lafayette P, Tucker D, Johnson S, Anderson J, Alaswad A, Cianzio S R, Parrott W A, Korkin D, Meksem K, Mitchum M G. Systematic mutagenesis of serine Hydroxy methyltransferase reveals an essential role in nematode resistance. Plant Physiology, 2017, 175: 1370-1380.
    [22] Meksem K, Pantazopoulos P, Njiti V N, Hyten L D, Arelli P R, Lightfoot D A. ‘Forrest’ resistance to the soybean cyst nematode is bigenic: saturation mapping of the Rhg1 and Rhg4 loci. Theoretical and Applied Genetics, 2001, 103: 710-717.
    [23] Niblack T L, Lambert K N, Tylka G L. A model plant pathogen from the kingdom animalia: Heterodera glycines, the soybean cyst nematode. Annual Review of Phytopathology, 2006, 44: 283-303.
    [24]Diers B W, Arelli P. Management of parasitic nematodes of soybean through genetic resistance. In: 1999: Proceedings of the World Soybean Research Conference, 6th; Aug. 4-7 1999; Chicago, IL. Chicago, IL: Superior Printing; 1999. p. 1300-1306.
    [25] 卢为国, 盖钧镒, 李卫东. 黄淮地区大豆胞囊线虫(Heterodera glycines Ichinohe)生理小种的抽样调查与研究. 中国农业科学, 2006, 39(2): 306-312.
    Lu W G, Gai J Y, Li W D. Sampling survey and identification of races of soybean cyst nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys. Scientia Agricultura Sinica, 2006, 39(2): 306-312. (in Chinese)
    [26] Vuong T D, Sleper D A, Shannon J G, Nguyen H T. Novel quantitative traitloci for broad-based resistance to soybean cyst nematode (Heterodera glycines Ichinohe) in soybean PI 567516C. Theoretical and Applied Genetics, 2010, 121: 1253-1266.
    [27] Shi Z, Liu S M, Noe J, Arelli P, Meksem K, LI Z L. SNP identification and marker assay development for high-throughput selection of soybean cyst nematode resistance. BMC Genomics, 2015, 16: 314.
    [28] Kadama S, Vunoga T D, Qiu A D, Meinhardta C G, Song L, Deshmukha R, Patila G, Wan J R, Valliyodana B, Scabooa A M, Shannonb J G, Nguyen H T. Genomic-assisted phylogenetic analysis and marker development for next generation soybean cyst nematode resistance breeding. Plant Science, 2016, 242: 342-350.
    [29] Riggs R D, Schmitt D P. Complete characterization of the race scheme for Heterodera glycines. Journal of Nematology, 1988, 20: 392-395.
    [30] Anand S C, Rao-Arelli A P. Genetic analyses of soybean genotypes resistant to soybean cyst nematode race 5. Crop Sci, 1989, 29: 1181-1184.
    [31] Arelli P R, Young L D, Concibido V C. Inheritance of resistance in soybean PI 567516C to LY1 nematode population infecting cv. Hartwig. Euphytica, 2009, 165: 1-4.
    [32] Guo B, Sleper D A, Arelli P R, Shannon J G, Nguyen H T. Identification of QTLs associated with resistance to soybean cyst nematode races 2, 3 and 5 in soybean PI 90763. Theor Appl Genet, 2005, 111: 965-971.
    [33] Vunong T D, Sleper D A, Shannon J G, Wu X, Nguyen H T. Confirmation of quantitative trait loci for resistance to multiple-HG types of soybean cyst nematode (Heterodera glycines Ichinohe). Euphytica, 2011, 181: 101-113.
    [34] Webb D M, Baltazar B M, Rao-Arelli A P, Schupp J, Clayton K, Keim P, Beavis W D. Genetic mapping of soybean cyst nematode race-3 resistance loci in the soybean PI 437654. Theoretical and Applied Genetics, 1995, 91: 558-574.
    [35] Concibido V C, Denny R L, Boutin S R, Hautea R, Orf J H, Young N D. DNA marker analysis of loci underlying resistance to soybean cyst nematode (Heterodera glycines Ichinohe). Crop Science, 1994, 34: 240-246.
    [36] Glover K D, Wang D, Arelli P R, Carlson S R, Cianzio S R, Diers B W. Near-isogenic lines confirm a soybean cyst nematode resistance gene from PI 88788 on linkage group. Journal of Crop Science, 2004, 44: 936-941.
    [37] Yue P, Sleper D A, Arelli P R. Mapping resistance to multiple races of Heterodera glycines in soybean PI 89772. Crop Science, 2001, 41: 1589-1595.
    [38] Bassuner R, Clayton K, Lewers K S, Mackenzie S, Shoemaker R C, Vaghchhipa Wala Z. Modulations in gene expression and mapping of genes associated with cyst nematode infection of soybean. Molecular Plant-Microbe Interaction, 2001, 14: 42-54.
    [39] Wang D, Diers B W, Arelli P R, Shoemaker R C. Loci underlying resistance to race 3 of soybean cyst nematode in Glycine soja plant introduction 468916. Theoretical and Applied Genetics, 2001, 103: 561-566.
    [40] Winter S M J, Shelp B J, Anderson T R, Welacky T W, Rajcan I. QTL associated with horizontal resistance to soybean cyst nematode in Glycine soja PI464925B. Theoretical and Applied Genetics, 2007, 114: 461-472.
    [41] Mahalingam R, Skorupska H T. DNA markers for resistance to Heterodera glycines. Race 3 in soybean cultivar Peking. Breeding Science, 1995, 45: 435-443.
    [42] Guo B, Sleper D A, Nguyen H T, Arelli P R, Shannon J G. Quantitative trait loci underlying resistance to three soybean cyst nematode populations in soybean PI 404198A. Crop Science, 2006, 46: 224-233.
    [43] Wu X, Blake S, Sleper D A, Shannon J G, Cregan P, Nguyen H T. QTL, additive and epistatic effects for SCN resistance in PI 437654. Theoretical and Applied Genetics, 2009, 118: 1093-1105.
    [44] Patil G, Do T, Vuong T D, Valliyodan B, Lee J D, Chaudhary J, Shannon J G, Nguyen H T. Genomic-assisted haplotype analysis and the development of high-throughput SNP markers for salinity tolerance in soybean. Scientific Reports, 2016, 6: 19199.
    [45] Tran D T, Steketee C J, Boehm J D Jr, Noe J, Li Z. Genome-Wide association analysis pinpoints additional major genomic regions conferring resistance to soybean cyst nematode (Heterodera glycines Ichinohe). Front Plant Science, 2019, 10: 401.
    [46] Zhao X, Teng W L, Li Y H, Liu D Y, Cao G L, Li D M, Qiu L J, Zheng H K, Han Y P, Li W B. Loci and candidate genes conferring resistance to soybean cyst nematode HG type 2.5.7. BMC Genomics, 2017,18: 462.
    [47] Liu S M, Ge F Y, Huang W K, Lightfoot D A, Peng D L. Effective identification of soybean candidate genes involved in resistance to soybean cyst nematode via direct whole genome re?sequencing of two segregating mutants. Theoretical and Applied Genetics, 2019, 132: 2677-2687.
    [48] Espindola S M, Hamawaki O T, Oliveira A P, Hamawaki C D, Hamawaki R L, Takahashi L M. Selecting soybean resistant to the cyst nematode Heterodera glycines using simple sequence repeat (microssatellite) markers. Genetic Molecular Research, 2016, 15: 15016850.
    [49] Fujino K, Hirayama Y, Kaji R. Marker-assisted selection in rice breeding programs in Hokkaido. Breed Science, 2019, 69: 383-392.
    [50] Kim K S, Vuong T D, Qiu D, Robbins R T, Shannon J G, Li Z L, Nguyen H T. Advancements in breeding, genetics, and genomics for resistance to three nematode species in soybean. Theoretical and Applied Genetics, 2016, 129: 2295-2311.
    [51] Young L D. Heterodera glycines populations selected for reproduction on Hartwig soybean. Journal of Nematology, 1998, 30: 523.
    [52] Braostowski L. Stacking alleles from multiple sources to increase broadspectrum genetic resistance to highly virulent soybean cyst nematode isolates. In: Proceedings of the 2016 Soybean Cyst Nematode Conference; 2016 Dec 13–15; Coral Gables, FL, USA. St. Paul: APS Press. 2016.
    [53] Yan G P, Baidoo R. Current research status of Heterodera glycines resistance and its implication on soybean breeding. Engineering, 2018, 4: 534–541.
    相似文献
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

练云,李海朝,李金英,等.利用KASP标记筛选含rhg1和Rhg4位点的大豆抗病资源[J].植物遗传资源学报,2021,22(2):399-406.

复制
分享
文章指标
  • 点击次数:1225
  • 下载次数: 1646
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2020-08-27
  • 最后修改日期:2021-01-18
  • 录用日期:2021-01-04
  • 在线发布日期: 2021-03-09
文章二维码
您是第5921211位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2025 版权所有
技术支持：北京勤云科技发展有限公司
请使用 Firefox、Chrome、IE10、IE11、360极速模式、搜狗极速模式、QQ极速模式等浏览器，其他浏览器不建议使用!