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镉胁迫下甜、糯玉米开花期性状的全基因组关联分析
作者:
作者单位:

1重庆市农业科学院,重庆,401329;2四川农业大学玉米研究所,成都, 611130;3福建省农业科学院作物研究所/福建省特色旱作物品种选育工程技术研究中心,福州,350013;4广东省农业科学院作物研究所,广州,510640;5浙江省东阳玉米研究所,东阳,322105;6四川省农业科学院生物技术核技术研究所,成都,610061

基金项目:

重庆市技术创新与应用示范项目：加工专用型高直链淀粉玉米材料创制及应用（cstc2018jscx-msyb0668）; 福建省属公益类科研院所专项（2018R1026-7；2019R1031-13）和福建省农业科学院科技创新团队项目（STIT2017-2-6）; 重庆市农业科学院农业发展资金项目: 玉米穗腐病抗源筛选鉴定与应用(NKY-2019AB014)


Genome Wide Association Analysis of Flowering Traits in Sweet and Waxy Maize under Cadmium Stress
Author:
Affiliation:

1Chongqing Academy of Agricultural Science,Chongqing,401329; 2Maize Research Institute of Sichuan Agricultural University,Chengdu,611130;3Crop Research Institute, Fujian Academy of Agricutural Sciences/Fujian Engineering Research Center for Characteristic Dry crop varieties Breeding, Fuzhou, 350013; 4Crop research Institute, Guangdong Academy of Agricultural Science,Guangzhou,510640; 5Zhejiang Academy of Agricultural Science,Dongyang,322105;6Biotechnology and Nuclear Technology Research Institute,Chengdu,610061

Fund Project:

The Project of Technology Innovation and Application Demonstration of Chongqing;The agricultural Development Fund Project of Chongqing Academy of Agricultural Sciences

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    摘要:

    本研究以183份甜、糯玉米自交系构成的关联群体为材料，在两种Cd污染水平下对甜、糯玉米开花期(Days to anthesis , DA) 、吐丝期(Days to silking ,DS)和开花吐丝间隔(Anthesis silking interval ,ASI)进行全基因组关联分析，以揭示重金属Cd胁迫对甜、糯玉米开花期的影响及调控基因。结果表明：不同Cd污染水平对甜、糯玉米DA和DS均产生影响，其中对DS的延长更为明显，从而导致ASI的增大。同时，甜玉米ASI平均值高于糯玉米ASI，由此表明甜玉米ASI对重金属Cd胁迫更为敏感。结合群体基因型和开花期相关性状表型的全基因组关联分析，分别筛选到3个开花期相关的SNP位点和6个吐丝期相关的SNP位点。根据其所对应的物理位置与Maize GDB和NCBI基因数据库进行比对，预测获得8个已知功能基因，其中包括在前人研究中与玉米开花期有关的候选基因。以上研究为解析甜、糯玉米在重金属Cd污染水平下开花期及吐丝期的遗传规律，为今后开展甜、糯玉米分子标记辅助育种和Cd安全品种的选育提供参考。

    Abstract:

    In this study, 183 sweet and waxy maize inbred lines were subjected to analyze the flowering time (Days to anthesis, DA), silking time (Days to silking, DS) and Anthesis silking interval (ASI) under Cd-polluted environments, in order to reveal the response and possible regulatory genes at the flowering stage. The results showed that Cd pollution prolonged DA and DS of sweet and waxy maize, especially for DS, which led to the increasing of ASI. The ASI value of waxy maize was found to be lower than that of sweet maize, implying the sensitivity of sweet corn under Cd stress. Three and six SNPs, which were associated with flowering time and silking time, respectively, were detected by Genome-wide association analysis (GWAS). These SNPs were found to be associated with eight genes annotated in maize GDB and NCBI gene databases, including few genes which were reported roles in the flowering time. Collectively, these results might provide theoretical reference for molecular marker assisted breeding and Cd safe breeding of sweet and waxy maize varieties in the future.

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袁亮,孟鑫,汪亚龙,等.镉胁迫下甜、糯玉米开花期性状的全基因组关联分析[J].植物遗传资源学报,2021,22(2):438-447.

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  • 收稿日期:2020-09-03
  • 最后修改日期:2020-11-03
  • 录用日期:2020-11-20
  • 在线发布日期: 2021-03-09
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