Southern rice black-streaked dwarf disease(SRBSDD), which is transmitted by white-backed planthopper [Sogatella furcifera (Horváth)], has caused severe yield loss in epidemic years in rice production. However, the reports on large-scale screening of SRBSDD resistant resources remain rare. In this study, 2812 Cultivated Rice Germplasm were evaluated for SRBSDD resistance under field and greenhouse conditions. The results showed that only 0.46% of tested accessions were resistance,the disease resistance level of different geographical sources, different germplasm types and different subspecies types was not consistent; 99.54% of tested accessions were susceptible, including many widespread varieties that are deploying in farming practice. That implied a potential of the increasing incidence and degree of SRBSDD in China in future. Seven genotypes showed reliable moderate disease resistance in multiple rounds of selection, with an average disease incident ranged from 6.72% to 28.51%. Specifically, avariation on heading date, plant height, effect panicle per plant, panicle length, spikelets per panicle, spikelet fertility, 1000-grain weight, single plant yield and length/width ratio of grain was observed in those 7 genotypes. Thus, this study unlocked the diseased incidence of SRBSDD in Cultivated Rice Germplasm, which will provide reference for rice resistance breeding.