Abstract:One wild strawberry accession, which was collected from Linxia city, Gansu Province is conserved in the National Clonal Germplasm Repository in Nanjing, China. In order to identify its taxonomy, the main taxonomical characters such as plant posture, leaflet type, stolon branching, flower sex, fruit shape and chromosome number have been investigated in the natural habitat (Linxia city, Gansu province). Plant (diploid, 2n=2x=14) is slender with trifoliate leaves (rarely quinquefoliolate); inflorescences are in cymes with 3 to 5 bisexual flowers per inflorescence; fruits are red, oval or short cylindrical; achenes are sunken; Calyxes are flat or slightly inversely curving; the runners are monopodial. This wild accession has been identified F.chinensis. In the natural habitat, plants generally germinate in March, bud appears in early May, and fruit ripens in mid-June. The floral bud differentiation has initiated since the middle of July (lasting for 60 days at 10 to 25°C), while 75% of floral bud have differentiated in the first 30 days. The leaves turned yellow in late September, withered in November before the dormancy. Based on the comparative analysis of climatic factors (Linxia and Nanjing), we discussed the environmental factors that associated to the flower bud differentiation of strawberry, as well as the reasons that resulted in a compromised flowering of F.chinensis in Nanjing.