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首页 > 过刊浏览>2021年第22卷第5期 >1304-1313. DOI:10.13430/j.cnki.jpgr. 20210316003 优先出版
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水稻穗异常突变体tutou4的鉴定及基因定位
DOI:
10.13430/j.cnki.jpgr. 20210316003
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作者:
  • 宋远辉

    宋远辉

    安徽农业大学农学院
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  • 花 芹

    花 芹

    安徽农业大学农学院
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  • 林泉祥

    林泉祥

    安徽农业大学农学院
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  • 郑思怡

    郑思怡

    安徽农业大学农学院
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  • 欧阳晨林

    欧阳晨林

    安徽农业大学农学院
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  • 杨 晔

    杨 晔

    安徽农业大学农学院
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  • 孙家猛

    孙家猛

    安徽农业大学农学院
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  • 陈庆全

    陈庆全

    安徽农业大学农学院
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  • 李金才

    李金才

    安徽农业大学农学院
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  • 张海涛

    张海涛

    安徽农业大学农学院
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作者单位:

安徽农业大学农学院

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(31871603, 31201205);国家留学基金委项目(201708340008);安徽省科技厅项目(KJ2017A143, gxfxZD2016022);安徽省科技重大专项(1803071176)


Identification and Fine Mapping of A Panicle Aberrant Mutant tutou4 in Rice (Oryza sativa L.)
Author:
  • SONG Yuan-hui

    SONG Yuan-hui

    College of Agronomy, Anhui Agricultural University
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  • HUA Qin

    HUA Qin

    College of Agronomy, Anhui Agricultural University
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  • LIN Quan-xiang

    LIN Quan-xiang

    College of Agronomy, Anhui Agricultural University
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  • ZHENG Si-yi

    ZHENG Si-yi

    College of Agronomy, Anhui Agricultural University
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  • OUYANG Chen-lin

    OUYANG Chen-lin

    College of Agronomy, Anhui Agricultural University
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  • YANG Ye

    YANG Ye

    College of Agronomy, Anhui Agricultural University
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  • SUN Jia-meng

    SUN Jia-meng

    College of Agronomy, Anhui Agricultural University
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  • Chen Qing-quan

    Chen Qing-quan

    College of Agronomy, Anhui Agricultural University
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  • Li Jin-cai

    Li Jin-cai

    College of Agronomy, Anhui Agricultural University
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  • ZHANG Hai-tao

    ZHANG Hai-tao

    College of Agronomy, Anhui Agricultural University
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Affiliation:

College of Agronomy, Anhui Agricultural University

Fund Project:

the National Natural Science Foundation of China (31871603, 31201205),China Scholarship Council (201708340008),Department of Education, Anhui Province (KJ2017A143,gxfxZD2016022),Anhui Province major special science and technology project (1803071176)

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    摘要:

    水稻穗异常退化现象在水稻的生长发育过程中经常发生。穗部退化可引起穗长、枝梗数、每穗粒数和结实率下降影响单株产量。穗退化性状因其遗传基础复杂、受环境和生理因素影响较大,其分子机制及其遗传网络仍知之甚少。tutou4是一个来自于组织培养的穗异常退化突变体,突变体表现为穗部部分颖花退化,总穗粒数和枝梗数减少,单株产量降低。遗传分析表明,该性状由一对隐性核基因控制,通过图位克隆的方法最终将候选基因定位在水稻8号染色体短臂端Os8-3-2和Os8-3-3之间,遗传距离为39.09 kb的范围内。该区间包括3个编码基因,通过测序发现tutou4突变体中基因LOC_Os08g06480的起始密码子上游有4325 bp的片段缺失,且最后一个外显子(7538 bp)上碱基A被替换成碱基G,但氨基酸未改变。荧光定量PCR的结果表明,该基因在突变体中表达量显著下降。因此,tutou4穂异常性状可能是由Tutou4启动子缺失或外显子碱基替换造成的基因表达量降低引起的表型突变。Tutou4为已报道基因OsLIS-L1/OsREL2/ASP1的等位基因。Tutou4表达量变化引起穗发育异常的表型为研究穗部发育的分子机制提供新的材料基础。

    关键词:水稻;穗异常;表型分析;花粉败育;图位克隆
    Abstract:

    Aberrant degeneration of panicle (ADP) usually occurs during the reproductive growth in rice. It causes yield loss due to the reductions of panicle length, branch number, grain number per panicle and seed setting. However, the ADP phenotype is sensitive to growth environments and regulated by multiple quantitative trait loci, which have complicated the identification of ADP-related function genes.The molecular mechanism underlying ADP occurrence remain largely unknown. The tutou4 is a panicle degeneration mutant derived from a tissue culture, and shows abnormal panicle, reduction of panicle grain number and branch number, as well as single plant yield. Genetic analysis showed that the ADP trait was controlled by a pair of recessive nuclear gene. The candidate gene was finally mapped to the short arm of chromosome 8, flanked by both Indel markers Os8-3-2 and Os8-3-3, with an interval of 39.09 kb, which contained three encoding genes. The sequencing result showed a 4325-bp deletion in the promoter region and one base alternation (A?G, synonymous) of the gene LOC_Os08g06480, which was designated as OsLIS-L1/OsREL2/ASP1. Real-time PCR analysis revealed a reduction on gene expression in mutant to that of the wild type. Considering the expression level decreased in the tutou4, the mutant will be useful germplasm in the study of abnormal panicle degeneration.

    Key words:rice; aberrant panicle; phenotypic analysis; pollen abortion; map-based cloning
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宋远辉,花 芹,林泉祥,等.水稻穗异常突变体tutou4的鉴定及基因定位[J].植物遗传资源学报,2021,22(5):1304-1313.

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  • 收稿日期:2021-03-16
  • 最后修改日期:2021-05-15
  • 录用日期:2021-06-22
  • 在线发布日期: 2021-09-07
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