The sequence analysis of CPP (cysteine-rich polycomb-like protein) gene family in mango (Mangifera indica) was carried out by bioinformatics methods, and the transcription level and tissue-specific expression of the CPP family genes during the infection of Xanthomonas campestris pv. mangiferaeindicae (Xcm) and Colletotrichum gloeosporioides (Cg) were studied using qRT-PCR. Ten members of the CPP gene family were identified from the mango genome, all of which contained conserved CRC domains, and they were all unstable hydrophobic proteins. A phylogenetic tree was built with 69 members of the CPP gene family from mango, Pyrus bretschneideri, periwinkle, Populus euphratica, rice, grape, and Arabidopsis thaliana, which were divided into 7 evolutionary branches. The 10 MiCPPs genes of mango were distributed in 5 of the evolutionary branches, most of which were clustered with members of the CPP gene family of Pyrus bretschneideri. qRT-PCR analysis showed that the relative expression levels of MiPP1 and MiCPP2 were up-regulated at 3 h, 6 h and 12 h after the Xcm infection, while the relative expression level of MiCPP8 was up-regulated and that of MiCPP9 was down-regulated at 0-72 h after the Cg infection. qRT-PCR analysis also showed that the relative expression levels of MiCPP8 were very high in young leaves, flowers and buds infected by Colletotrichum gloeosporioides, while the relative expression levels of MiCPP7 were very low in those parts, the relative expression levels of MiCPP4 and MiCPP9 were high in young leaves and flowers but very low in buds, and the relative expression of MiCPP6 was very low in flowers. These results indicated the expression specificity of the mango MiCPPs gene family members in different tissues. This research would provide a basis for further studies on the response mechanism of mango CPP gene family members to pathogen infection and help breed for disease resistance in mango.