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首页 > 过刊浏览>2021年第22卷第6期 >1723-1731. DOI:10.13430/j.cnki.jpgr. 20210421002
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基于BSA-seq法的纤维衣分相关候选基因的鉴定
作者:
作者单位:

1山西农业大学农学院,晋中 030801;2 安阳工学院生物与食品工程学院,安阳 455000

基金项目:

国家自然科学基金（U1804103）; 棉花生物学开放基金国家重点实验室（CB2020A10）; 山西农业大学博士科研启动项目（2020BQ46)


Identification of Candidate Genes Associating with Fiber Lint Percentage Using BSA-seq
Author:
Affiliation:

1College of Agriculture,Shanxi Agricultural University,Jinzhong 030801; 2 School of Biological and Food Engineering,Anyang Institute of Technology,Anyang 455000

Fund Project:

National Natural Science Foundation of China (U1804103)， Sponsored by State Key Laboratory of Cotton Biology Open Fund (CB2020A10），Doctoral research project of Shanxi Agricultural University (2020BQ46)

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    摘要:

    衣分是影响棉纤维产量的重要指标。本研究以高衣分棉花陆海渐渗系材料MBI7747-14和中棉所45为亲本，构建包含2403个单株的F 2分离群体，挑选衣分性状极端单株构建两个混池，采用BSA-seq方法进行衣分相关基因定位分析。结果表明，在D 02染色体上得到四个置信指数高于95%的候选区间，四个区间共包含236个基因，总长度为5.47 Mb。在以上基因中，200个基因中含有SNP，190个基因中含有InDel，其中70个基因含有非同义突变位点。通过转录组数据的基因表达模式分析，筛选出19个可能与衣分相关的候选基因。GO功能富集分析表明，19个候选基因集中于NADP+活性、醛糖醇代谢、碳利用和调控细胞发育等功能条目；KEGG分析结果表明，19个候选基因主要参与ABC转运过程和苯丙烷生物合成途径。本研究为进一步研究棉纤维衣分形成的遗传机制奠定了一定的基础。

    关键词:衣分;BSA-seq;QTL
    Abstract:

    Lint percentage is an important index affecting cotton fiber yield. In this study, an F2 segregation population containing 2403 individual plants was constructed derived from chromosome introgression line MBI 7747-14 (Gossypium barbadense x Gossypium hirsutum) with high lint percentage crossing with CCRI 45. The BSA-seq was conducted using two bulked samples by pooling individual plants showing higher and lower lint percentage, respectively. As a result, four candidate physical regions showing confidence indices higher than 95% were obtained in a total length of 5.47 Mb on chromosome D02, which contained 236 annotated genes. Out of them, 200 genes contain SNPs, 190 genes contain Indel, and 70 genes contain non-synonymous mutation sites. Through gene expression pattern analysis of transcriptome data, 19 candidate genes possibly associating with lint percentage were identified. GO functional enrichment analysis showed that the 19 candidate genes were enriched in NADP+ activity, aldol metabolism, carbon utilization and regulation of cell development. KEGG analysis showed that the 19 candidate genes were mainly involved in pathway such as ABC transport and phenylpropane biosynthesis. Collectively, this study laid a foundation for further analyzing the genetic mechanism of cotton fiber lint percentage formation.

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赵岩,冯加加,张丽,等.基于BSA-seq法的纤维衣分相关候选基因的鉴定[J].植物遗传资源学报,2021,22(6):1723-1731.

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  • 收稿日期:2021-04-21
  • 最后修改日期:2021-05-14
  • 录用日期:2021-06-15
  • 在线发布日期: 2021-11-04
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