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首页 > 过刊浏览>2022年第23卷第1期 >72-82. DOI:10.13430/j.cnki.jpgr.20210427002
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不同来源大麦对条纹病抗性鉴定及遗传多样性分析
作者:
作者单位:

1.甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室;2.甘肃省农民教育培训工作总站;3.陇东学院农林科技学院;4.浙江省农业科学院作物与核技术利用研究所;5.甘肃农业大学农学院

基金项目:

财政部和农业农村部：国家现代农业产业技术体系资助（CARS-05-04B-2）；甘肃省教育厅产业支撑计划项目（2021CYZC-12）；国家自然基金（31960426）；国家自然基金（32160496）；甘肃省科技重大专项计划（17ZD2NA016）；甘肃省青年科技基金计划（20JR5RA010）；甘肃农业大学科技创新基金-公招博士科研启动基金（GSAURCZX201706）；甘肃农业大学学科建设专项基金（GAU-XKJS-2018-082、083）；甘肃省科技支撑计划项目（1604NKCA052）；甘肃省教育厅创新能力提升项目（2019A-053）；甘肃省教育厅创新基金项目（2021A-055）


Resistance Identification and Genetic Diversity Analysis of Barley Genotypes from Different Sources to Barley Stripe Disease
Author:
Affiliation:

1.Gansu Provincial Key Lab of Aridland Crop Science / Gansu Provincial Key Lab of Crop Improvement and Germplasm Enhancement;2.Gansu Provincial Farmer Education and Training Station;3.College of Agriculture and Forestry, Longdong University, Gansu Qingyang;4.Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences;5.College of Agronomy, Gansu Agricultural University

Fund Project:

China Agriculture Research System of MOF and MARA(CARS-05-04B-2), Industrial Support Project of Gansu Provincial Education Department(2021CYZC-12), National Natural Science Foundation(31960426), National Natural Science Foundation(32160496), Major Science and Technology Special Program of Gansu Province (17ZD2NA016), Science and technology Fund Project of Gansu Provincial(20JR5RA010), Science and technology innovation fund of Gansu Agricultural University- Public doctoral research fund (GSAURCZX201706), Subject Construction Special Fund of Gansu Agricultural University (GAU-XKJS-2018-082, 083), Gansu Science and Technology Support Program (1604NKCA052), Innovation ability improvement project of Gansu Provincial Department of Education (2019A-053), Innovation Fund Project of Gansu Provincial Department of Education(2021A-055)

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    摘要:

    为了解不同来源大麦对条纹病的抗性及遗传多样性，本研究采用三明治法通过人工接种大麦条纹病菌对91份大麦材料进行抗性评价，并通过31个SSR 标记对91份大麦亲本材料进行遗传多样性和群体结构分析。结果表明，人工接种大麦条纹病菌后共鉴定出4份免疫、6份高抗、33份抗病、42份感病和6份高感大麦亲本材料；31对SSR标记共检测出等位基因238个，平均每对标记可检测到7.677个等位基因，等位基因的变幅为2～19；主基因频率变化范围为0.236～0.951，平均值为0.394；基因多样性指数的变幅为0.094～0.871，平均值为0.667；多态性信息量变幅为0.091～0.860，平均值为0.613；遗传相似系数变异范围为0.103～1.000，平均值为0.522；在GS值为0.783水平上可将参试材料聚为3个大类群，各大类分别包含86、2和3份材料；群体遗传结构分析表明，供试大麦亲本材料分为3个亚群，每个亚群分别包含47、33和32份材料，且在91份材料中，Q＞0.6的材料占总数的97.80%。本研究经抗病鉴定及分子标记结果综合分析，可为挑选抗病亲本辅助抗大麦条纹病优良品种的选育提供参考。

    Abstract:

    In order to detect the resistance genes of germplasm resources against barley stripe disease and understand their genetic diversity, in this study 91 barley accessions were inoculated with barley stripe disease pathogen by sandwich method, followed by the genetic diversity analysis using 31 polymorphic SSR markers. Of them, four accessions were identified to be complete resistance (free of diseased symptom), 6 highly resistant, 33 resistant, 42 susceptible and 6 high susceptible. Genotyping by 31 SSR markers disclosed 238 alleles, with a mean of 7.677 alleles in each of markers and the range of 2 to 19 alleles. The frequency of major loci ranged from 0.236 to 0.951, with an average of 0.394. The gene diversity index ranged from 0.094 to 0.871, with an average of 0.667. PIC ranged from 0.091 to 0.860, with an average of 0.613, while GS ranged from 0.103 to 1.000, with an average of 0.522. This collection were divided into three groups at the genetic similarity coefficient level of 0.783, each containing 86, 2 and 3 accessions, respectively. The population structure analysis suggested three groups, each including 47, 33 and 32 materials, respectively. 97.80% of 91 genotypes had Q value greater than 0.6. Gained from disease resistance identification and genotyping, this study provided reference for selecting parental lines in breeding of barley leaf stripe resistant varieties.

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郭铭,张金福,司二静,等.不同来源大麦对条纹病抗性鉴定及遗传多样性分析[J].植物遗传资源学报,2022,23(1):72-82.

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  • 收稿日期:2021-04-27
  • 最后修改日期:2021-08-16
  • 录用日期:2021-09-02
  • 在线发布日期: 2022-01-07
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