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首页 > 过刊浏览>2022年第23卷第3期 >811-822. DOI:10.13430/j.cnki.jpgr.20211203002
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小麦不育小穗数 QTL-qSsnps-5D 遗传及育种选择效应解析
作者:
作者单位:

1.鲁东大学农学院 / 山东省高等学校作物高产抗逆分子模块重点实验室;2.聊城市农业科学院现代育种技术中心;3.山东省农业技术推广中心

基金项目:

山东省重点研发计划（2019GNC106126）；国家自然科学基金（31871612）；山东省高等学校“青创科技计划”（2019KJF002）；烟台 市科技计划项目（2020XCZX045）


Genetic Effect of Sterile Spikelet Number-related QTL-qSsnps-5D and Its Use in Wheat Varieties
Author:
Affiliation:

1.School of Agriculture,Ludong University/Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong;2.Modern Breeding Technology Center,Liaocheng Academy of Agricultural Sciences,Shandong Liaocheng;3.Shandong Agricultural Technology Extension Center

Fund Project:

Shandong Provincial Key Research and Development Program（2019GNC106126），National Natural Science Foundation of China（31871612），Youth Innovation Science and Technology Program of Colleges and Universities in Shandong Province （2019KJF002），Yantai Science and Technology Planning Project（2020XCZX045）

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    摘要:

    qSsnps-5D 为一个控制不育小穗数的主效稳定 QTL，其优异等位基因来自小麦骨干亲本京 411。本研究利用科农 9204× 京 411 衍生的包含 187 个家系的重组自交系群体（KJ-RIL，recombinant inbred lines derived from the cross of Kenong 9204 and Jing 411）及 314 份育成品种（系）组成的自然群体对其进行遗传及育种选择效应解析，明确其对产量性状的遗传 效应，分析其在育种过程中的选择应用情况，评价其未来育种应用潜力。试验结果表明，qSsnps-5D 在 8 套数据集中被定位 于 5D 染色体上 0.72~4.13 Mb 之间，跨度约 3.41 Mb。基于 KJ-RIL 群体及自然群体分析结果均表明，来自京 411 的优异等位 基因可增加单株穗数，但对千粒重表现为极显著负向效应；其对穗粒数、单株产量的影响在两套群体的分析结果不一致。在 qSsnps-5D 靶区间内选择 2 个紧密连锁的 SNP 标记 AX-110565536 和 AX-86170796 对 314 份自然群体进行目标 QTL 单倍型 分析；结果显示，国外品种对 qSsnps-5D 优异单倍型（Hap-GG-CC）的选择利用率最高；中国品种中青海省、四川省和河南省 3 个省份优异单倍型品种占比较高，而山东、北京、陕西和河北 4 地对 qSsnps-5D 优异单倍型选择利用率较低。时间跨度显 示，qSsnps-5D 优异单倍型 Hap-GG-CC 选择利用效率随时间推移在我国呈下降趋势。为便于 qSsnps-5D 后期分子育种应用， 本研究开发了一个基于 PCR 检测技术的 InDel 分子标记，命名为 5D-1620921，其带型扩增清晰，可重复性好，为 qSsnps-5D 分 子育种应用提供理论支撑。

    Abstract:

    qSsnps-5D，derived from the backbone parental line Jing 411（J411），is a major stable QTL for sterile spikelet number per spike（SSNPS）. This study aimed to decipher the genetic effect of this QTL and its use in wheat varieties. Two populations，including a recombinant inbred lines derived from the cross of Kenong 9204（KN9204）and Jing 411（KJ-RIL），and a natural mapping population comprised by 314 wheat authorized varieties or advanced lines，were involved. The experiment results showed that qSsnps-5D was confirmed in an interval of 3.41 Mb from 0.72 Mb to 4.13 Mb on chromosome 5D among 8 datasets. Excellent allele of qSsnps5D from J411 could increase spikes per plant but significantly decrease thousand kernel weight in both KJ-RIL and the natural mapping populations. However，the genetic effects of qSsnps-5D on kernel number per spike and yield per plant were differing between the two populations. Two close linkage markers of qSsnps-5D，i.e.，AX110565536 and AX-86170796 were used to specify the breeding utilization characteristics of qSsnps-5D in the natural mapping population. The excellent haplotype for qSsnps-5D（Hap-GG-CC）was often found（80.60%） in the foreign varieties. Among the Chinese varieties，higher rates of excellent haplotype（Hap-GG-CC）were found from varieties of Qinghai province，Sichuan province and Henan province，but the rates of haplotype HapGG-CC were lower in varieties of Shandong，Beijing，Shaanxi and Hebei. The rates of Hap-GG-CC in varieties released from 1980 s to present were constantly declined. To facilitate the application of qSsnps-5D in future molecular breeding programs，we developed a PCR-based InDel molecular marker named as 5D-1620921. Altogether，this study provided insights for the application of qSsnps-5D in molecular breeding programs of wheat.

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马天航,蔡益彪,熊永星,等.小麦不育小穗数 QTL-qSsnps-5D 遗传及育种选择效应解析[J].植物遗传资源学报,2022,23(3):811-822.

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  • 收稿日期:2021-12-03
  • 最后修改日期:2021-12-13
  • 录用日期:2021-12-21
  • 在线发布日期: 2022-05-10
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