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首页 > 过刊浏览>2022年第23卷第4期 >1037-1045. DOI:10.13430/j.cnki.jpgr.20211209001
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标记辅助创制富集花青素高油酸的花生种质
作者:
作者单位:

1.河北农业大学农学院;2.河北工程大学园林与生态工程学院;3.河北易园生态农业科技有限公司

基金项目:

河北省高等学校科学技术研究项目（ZD2019051）；河北省重点研发计划项目现代种业科技专项（19226363D）；2021 年度河北省保定市农业科技园区建设项目（2111N004）


Marker-Assisted Generation of High-oleic Germplasm Accessions Enriched with Anthocyanins in Peanut (Arachis hypogaea L.)
Author:
Affiliation:

1.College of Agronomy, Hebei Agricultural University;2.School of Landscape and Ecological Engineering,Hebei University of Engineering;3.Hebei Yiyuan Ecological Agriculture Technology Co, Ltd

Fund Project:

Key Project of Science and Technology Research in Colleges and Universities of the Department of Education in Hebei Province (ZD2019051)，Key Project of Science and Technology Research of Modern Seed Industry of the Department of S&T in Hebei Province (19226363D)，Project of Agricultural Science and Technology Park Construction in Baoding City, Hebei Province (2111N004)

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    摘要:

    花生（Arachis hypogaea L.）油酸和花青素含量是花生品质育种的重要目标。本研究以高油酸粉色种皮“G110”（G，♀）和普通油酸紫色种皮“紫珍珠”（Z，♂）组配杂交组合。亲本子仁在开花后30天（30 DAF，G1/Z1）和45 DAF（45 DAF，G2/Z2）的转录组分析结果表明，氧化-还原过程（GO:0055114）和脂肪酸合成过程（GO:0006633）为主要的GO富集代谢通路。差异基因表达结果表明，ahFAD2B（arahy. 5913QL）在G110中显著上调表达，ahFAD2A（arahy. 42CZAS）差异水平不显著。AlleleXa/b和AlleleYa/b分型结果表明，G110基因型为aabb、紫珍珠基因型为AABB。利用Kompetitive Allele-Specific PCR（KASP）荧光标记A004807和A004808，在F2筛选出aabb高油酸单株66株。经继代繁育后，在F7得到紫色种皮高油酸种质材料3个，分别为18-B-40、18-B-49和18-B-54，其油酸含量为分别为79.46%、78.77%和78.09%，分别是紫珍珠的1.77倍（p=3.61×10-9）、0.99倍（p=1.21×10-9）和0.99倍（p=1.45×10-9）；油亚比（O/L）分别为14.69、11.89和10.88，分别是紫珍珠的11.58倍（p=4.01×10-15）、9.37倍（p=7.92×10-15）和8.58倍（p=4.51×10-15）；花青素含量分别为30.20 OD/g、28.77 OD/g和29.13 OD/g，分别是G110的23.91倍（p=1.17×10-7）、22.77倍（p=4.00×10-10）和23.06倍（p=1.63×10-10）。本研究获得的富集花青素高油酸花生种质材料对丰富我国高油酸花生种质资源具有重要的现实意义，同时对花生油酸代谢机制的深入研究提供参考。

    Abstract:

    The content of oleic acid and anthocyanin is an important target for quality breeding in peanut (Arachis hypogaea L.). In this study, a high-oleic acid peanut line with pink testa "G110" (G, ♀) was crossed with a landrace line with purple testa "Purple Pearl" (Z, ♂). Kernels of both parent lines on 30 days after flowering (30 DAF, G1/Z1) and 45 DAF (G2/Z2) were sampled for the transcriptomic analysis. The differentially expressed genes (DEGs) enrichments in oxidation-reduction process (GO:0055114) and fatty acid biosynthetic process (GO:0006633) were observed. The gene ahFAD2B (arahy.5913QL) was up-regulated in G110, while another gene ahFAD2A (arahy. 42CZAS) was not significant difference (p > 0.05). AlleleXa/b and AlleleYa/b genotyping results showed that “G110” was aabb and "Purple Pearl" was AABB. By taking use of kompetitive allele-specific PCR (KASP) markers A004807 and 4004808, 66 high-oleic acid plants of genotype aabb in F2 populations were obtained, followed by self-pollination to F7. Of them three superior accessions namely 18-B-40, 18-B-49 and 18-B-54, with purple testa and high-oleic acid were identified, in which the oleic acid content was 79.52%, 78.84%, and 78.02%, 1.77 folds (p=3.61×10-9), 0.99 folds (p=1.21×10-9) and 0.99 folds (p=1.45×10-9) higher than that of "Purple Pearl", respectively. The oleic to linoleic ratio (O/L) was 14.69, 11.91, and 10.90, which increased by 11.58 folds (p=4.01×10-15), 9.37 folds (p=7.92×10-15) and 8.58 folds (p=4.51×10-15) compared with that of "Purple Pearl". The anthocyanin content was 30.87 OD/g, 29.16 OD/g, and 14.51OD/g, which increased by 23.91 folds (p=1.17×10-7), 22.77 folds (p=4.00×10-10) and 23.06 folds (p=1.63×10-10) compared with that of "G110". Collectively, this study obtained peanut germplasm accessions showing simultaneous enrichments of anthocyanin and high oleic, which might have implications for enriching high oleic peanut germplasms in China and future uncovering the mechanism of peanut oleic acid metabolism.

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李佳伟,马钰聪,李 丽,等.标记辅助创制富集花青素高油酸的花生种质[J].植物遗传资源学报,2022,23(4):1037-1045.

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  • 收稿日期:2021-12-09
  • 最后修改日期:2022-03-23
  • 录用日期:2022-04-24
  • 在线发布日期: 2022-07-08
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