The tassel branch number of maize is one of the important factors affecting the yield production. Unlocking the QTL loci to control the tassel branch number of maize is of great significance for maize variety improvement and molecular assisted breeding. In this study，the individuals of a BC1F1 population derived from the parents Jing724 and Jing92 of Jingke968 were genotyped using high-throughput high-quality SNP array Maize6H-60K. By taking use of 28910 polymorphic SNPs，a high density genetic map containing 2737 BIN markers was constructed. The number of BIN markers on each chromosome ranged from 145 to 512， and the average genetic distance was 0.56 cM. Both parents and 727 offspring lines were planted in Beijing in 2021 to investigate the tassel branch number，followed by QTL mapping using Inclusive Composite Interval Mapping（ICIM） in software QTLIciMappingV4.2. A total of 6 QTL were detected，located on chromosomes 2，5，6，7，8 and 9，respectively. The LOD score of QTL ranged from 3.18 to 11.08，revealing 1.58% to 5.59% phenotypic variation. While four QTL were located in the same or adjacent area as previously reported，two novel QTL qTBN6 and qTBN7 have been revealed. The LOD of qTBN6 was 6.73，and the synergistic allele derived from Jing724，with a negative additive effect to reduce the tassel branch number. Collectively， this study laid a foundation for future isolating the functional genes that regulate the tassel branch number in maize.