Abstract:The soybean variety Heike 56 was selected from the offspring of the hybrids of Heihe 33 (female parent) and Heihe 34 (male parent) in Heihe Branch of Heilongjiang Academy of Agricultural Sciences through sexual hybridization and genealogy. In the adaptive cultivation zone, this variety would take 105 to 110 days from seedling emergence to maturity, with the effective accumulated temperature (≥10℃) of 2030 to 2100℃. This variety shows the following phenotypic characters: sublimited pod habit, plant height 69.6 to 75.0 cm, branched, white flowers, pointed leaves, gray fuzz, pod sickle shape, brown when mature; round seeds, yellow seed coat, light yellow seed hilum, shiny, 100-seed weight 16.5 to 19.0g; protein content 38.80 to 43.05%, and fat content 18.18 to 19.69% (mean value of 5 years datasets); resistance against gray spot disease (grade 2, R), and moderate resistance against viral disease (grade 3, MR). It is qualified to be cultivated in the lower limit of the fifth temperate zone and the upper limit of the sixth temperate zone in Heilongjiang Province. Through pedigree analysis, its cytoplasmic components was derived from ‘Keshan Baimei’, through 9 transmission processes in 12 rounds of breeding from the 3rd to the 12th (Keshan Baimei→ Zihua 4 → Harvest 1 → Heihe 54 → Heihe 4 → Heijiao 7710 → Heihe 9 → Heihe 18 → Heihe 33 → Heike 56). Its nuclear genetic components might be traced back to 14 ancestral parents (two excellent foreign germplasms, five backbone local varieties from northeast China, four locally breeding lines, and one wild soybean germplasm), with the contribution rates: ‘Bei 92-28’ (25.00%), ‘Shi Sheng Changye’ (15.36%), ‘Heijiao 83-1345’ (7.81%), ‘Yubilei’ (7.81%), ‘Heihe 51 (L)’ (6.25%), ‘Suoyiling’ (4.69%), ‘Keshan Baimei’ (4.30%), ‘Keshan Silijia’ (3.91%), ‘Silihuang’ (3.71%), ‘Jinyuan’ (3.71%), ‘Heihe Glycine Soja 3-A’ (3.13%), ‘Hejiao 75-861’ (3.13%), ‘Heilongjiang 41’ (3.13%), ‘Changye 1’ (3.13%). Collectively, the abundant genetic diversity of ‘Heike 56’ has laid the genetic foundation for the high and stable yield, high quality, strong stress resistance and super-wide adaptability.