Celery flowers, with the characteristics of small organ, many flowers, and in-consistent flowering time and small seeds, are considered as limits in cross breeding and seed production. The application of complete male sterile materials might bring the positive significance in celery breeding and seed production market. In this study, the genotype 'Jinnan celery' was mutagenized by radiation mutation in 2017, and a male sterile material (named: QCBU-001) was identified. By conducting the field trails over several years, this genotype showed completely male sterile without pollens, and maintained full function to pollinate with foreign pollens and produce seeds. With the preliminary results, the hybrid seeds were able to germinate and grow normally, with an obvious hybridization advantage. Sequencing of the mitochondrial genome in 'QCBU-001' enabled an assembly of 260,772 bp, containing 52 annotated mRNAs, 21 tRNAs and 7 rRNAs. The sequence analysis in released sequences of the celery sterile material 'W99A' and fertile material "W99B", as well as the mitochondrial gene cloned from 'Jinan Shiqin' revealed that 'QCBU-001' sterility was caused by Cox 1 mutation. Therefore, 'QCBU-001' is CMS male sterile material of celery, which can be used in celery cross breeding and seed production.