In order to identify kenaf germplasm resources resistant to root knot nematode， In this study， the root knot nematode population infected with kenaf was identified by SCAR primers， and the root knot nematode resistance of 220 kenaf wild or cultivated germplasm resource materials were identified and evaluated by using the methods of natural field disease and pot inoculation identification. The results showed that the pathogen causing disease in the field was Meloidogyne incognita. The results of field resistance identification showed that there were 133 kenaf resources showed high resistance， accounting for 60.45%， and all the roselle， false roselle and creeping saxifrage showed high resistance， and the high resistance ratio was the highest. The results of pot inoculation showed that there were 7 immune resources， accounting for 3.18% of the total resources. And 98 high resistance resources，accounting for 44.55% of the total resources. All roselle were identified as high resistance grade. There were some differences in the identification of kenaf germplasm resistance between natural field disease and pot test. There were 98 germplasm resources with the same identification results by the two methods.There were 69 high resistance resources ，accounting for 70.41% of the 98 germplasm resources. Among the high-resistance resources， 49 were from cultivated kenaf， 10 were from wild relatives， the rest were from roselle， rose hemp and hibiscus， and 1 high susceptible variety was from wild relatives.These results of this study provided scientific basis for the exploitation and utilization of kenaf germplasm resources against root knot nematode disease.