宁夏春麦区小麦资源株高、籽粒性状及抗病性分析
作者:
作者单位:

宁夏大学农学院,银川 750021

作者简介:

研究方向为小麦遗传育种,E-mail: sungang2812@163.com

通讯作者:

王掌军,研究方向为小麦遗传育种,E-mail: wangzj-gs@126.com

中图分类号:

基金项目:

国家自然科学基金项目(32160452);宁夏农业育种专项(2018NYYZ02);宁夏自然科学基金项目(2021AAC03069)


Analysis of Wheat Resources for Plant Height, Grain Traits, and Diseases Resistance Cultivated in Ningxia Spring Wheat Region
Author:
Affiliation:

Agricultural College, Ningxia University, Yinchuan 750021

Fund Project:

Foundation projects: National Natural Science Foundation Project of China(32160452);Ningxia Hui Autonomous Region Agricultural Breeding Special Project(2018NYYZ02);Ningxia Natural Science Foundation Project(2021AAC03069)

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    摘要:

    为评价我国4个主要育种阶段来源于不同麦区的小麦资源在宁夏春麦区的株高、籽粒性状和抗病性,以及为宁夏小麦育种提供可行的评价方法和优异种质,本研究以4个育种阶段共228份小麦资源为材料,采用熵值赋权DTOPSIS法对这些资源的株高和籽粒性状进行综合评价,以筛选优良育种亲本。通过田间抗性调查结合18个与宁夏小麦主要病害相关的分子标记对这些资源的抗病性及抗病基因分布情况进行评价。结果表明,从阶段Ⅰ~Ⅳ,材料的株高和蛋白质性状呈降低趋势,而穗粒数和粒重呈增加趋势。根据Ci值筛选出11份资源(占总数5%),分别为红兴隆3号、糯麦、新疆小麦、上林小麦、宁春45号、会宁19号、宁春32号、宁春58号、甘春25号、永良15号和小冰33号。抗性调查结果表明,228份资源中分别有181份、220份和83份表现抗条锈病、叶锈病和白粉病。Ci值筛选出的11份资源对条锈病和叶锈病表现出较好的抗性,其中红兴隆3号、上林小麦和小冰33号还兼抗白粉病。此外,18个与抗病基因相关的分子标记在228份资源中表现出不同比例的扩增。总之,基于熵值赋权的DTOPSIS法和小麦主要病害相关分子标记检测可用于国内小麦资源不同育种阶段的评价,筛选出的综合性状优良的小麦资源可作为宁夏小麦育种的候选亲本。

    Abstract:

    This study attempted to evaluate the plant height, grain traits, and diseases resistance of wheat resources derived from different wheat regions of China at four major breeding stages that were cultivated in spring wheat region of Ningxia Hui Autonomous region, China, in order to provide the methods and excellent germplasms for local wheat breeding in future. A total of 228 wheat resources at four breeding stages were used. The entropy-weighted DTOPSIS method was used for evaluating the plant height and grain traits in order to select good breeding parents. The diseases resistance and proportion of resistant genes were evaluated by field resistance survey, in combination with 18 molecular markers that associate with main wheat diseases in Ningxia, China. The results showed that, at the breeding stages I to IV, the plant height and index of the protein traits were gradually decreased, while the grain number and grain weight were gradually increased. According to the Ci value, 11 resources (Hongxinglong No.3, Nuomai, Xinjiang wheat, Shanglin wheat, Ningchun No.45, Huining No.19, Ningchun No.32, Ningchun No.58, Ganchun No.25, Yongliang No.15 and Xiaobing No.33) ranking top 5% in popupalation were identified. The resistance survey results showed, there were 181, 220 and 83 in 228 resources that were resistant to stripe rust, leaf rust and powdery mildew, respectively. These 11 resources based on the Ci value showed better resistance to stripe rust and leaf rust, and three of them, namely Hongxinglong No.3, Shanglin wheat, and Xiaobing No. 33, were also resistant to powdery mildew. In addition, 18 molecular markers associated with diseases resistance, showed different proportions of amplification results in 228 resources. Collectively, the DTOPSIS method based on entropy weighting and molecular markers associated with main wheat diseases can be used to evaluate the wheat resources at the different breeding stages.These selected wheat resources with good performance can be used as candidate parents for wheat breeding in Ningxia, China.

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孙刚,陈佳静,姚明明,等.宁夏春麦区小麦资源株高、籽粒性状及抗病性分析[J].植物遗传资源学报,2023,24(4):954-971.

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  • 收稿日期:2022-11-18
  • 最后修改日期:2022-12-28
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  • 在线发布日期: 2023-06-13
  • 出版日期: 2023-06-14
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