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首页 > 过刊浏览>2023年第24卷第4期 >1114-1121. DOI:10.13430/j.cnki.jpgr.20221212001 优先出版
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水稻开颖突变体的遗传分析和基因克隆
DOI:
10.13430/j.cnki.jpgr.20221212001
CSTR:
作者:
  • 毛兴学

    毛兴学

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 郑晓钰

    郑晓钰

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 潘大建

    潘大建

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 孙炳蕊

    孙炳蕊

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 江立群

    江立群

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 张静

    张静

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 吕树伟

    吕树伟

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 于航

    于航

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 李晨

    李晨

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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  • 陈文丰

    陈文丰

    广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640
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作者单位:

广东省农业科学院水稻研究所 / 农业农村部华南优质稻遗传育种重点实验室(部省共建) / 广东省水稻育种新技术重点实验室 / 广东省 水稻工程实验室,广州 510640

作者简介:

研究方向为水稻种质资源,E-mail: maoxingxue@qq.com

通讯作者:

陈文丰,研究方向为水稻遗传育种,E-mail: 18-cwf@163.com

中图分类号:

基金项目:

广东省自然科学基金(2019A1515011208);广东省重点领域研发计划项目(2020B0202090003,2022B0202110003);广东省农业科学院农业优势产业学科团队建设项目(202101TD);广东省学科类重点实验室运行项目(2020B1212060047);2022年省级乡村振兴战略专项资金(2022-NBH-00-012)


Genetic Analysis and Gene Cloning of an Open-hull Mutant in Rice
Author:
  • MAO Xing-xue

    MAO Xing-xue

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • ZHENG Xiao-yu

    ZHENG Xiao-yu

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • PAN Da-jian

    PAN Da-jian

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • SUN Bing-rui

    SUN Bing-rui

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • JIANG Li-qun

    JIANG Li-qun

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • ZHANG Jing

    ZHANG Jing

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • LYU Shu-wei

    LYU Shu-wei

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • YU Hang

    YU Hang

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • LI Chen

    LI Chen

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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  • CHEN Wen-feng

    CHEN Wen-feng

    Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640
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Affiliation:

Rice Research Institute, Guangdong Academy of Agricultural Science/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640

Fund Project:

Foundation projects: Guangdong Provincial Natural Science Foundations (2019A1515011208); The Key Field Research and Development Projects of Guangdong Province(2020B0202090003, 2022B0202110003); Agricultural Competitive Industry Discipline Team Building Projects of Guangdong Academy of Agricultural Sciences (202101TD); Projects of Guangdong Key Laboratory(2020B1212060047); Special Funds for Guangdong Provincial Rural Revitalization Strategy in 2022 (2022-NBH-00-012)

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    摘要:

    花器是种子生成的基础,水稻花器发育直接影响稻谷产量与品质。源美丝苗与P704杂交后代中发现一株开颖突变体(oh,open-hull),其穗部发育有缺陷,主要表现为:开颖,结实率显著下降,种子变小。遗传分析发现开颖表型由一对隐性细胞核基因控制。通过BSA分析,开颖基因被定位于水稻第3染色体上,检索发现候选区间与OsMADS1(LOC_Os03g11614)基因重合,与蜀恢498参考基因序列进行比对,oh中OsMADS1的第一外显子第118位发生碱基颠换,导致编码的天冬氨酸变成酪氨酸,将该等位基因命名为OsMADS1oh。从oh中克隆OsMADS1oh并构建过表达载体,通过农杆菌介导转入易转化材料(中花11)中,转基因后代出现开颖表型,证实OsMADS1oh导致开颖。另外,RNA定量分析结果表明,MADS8和YABB5的表达受OsMADS1调控。本研究从育种材料中筛选到开颖突变体,并从中鉴定了新的OsMADS1等位基因,为阐明花器形成的分子机理研究提供了特异种质和基因资源。

    关键词:水稻(Oryza sativa L.);开颖突变体;基因克隆
    Abstract:

    Floral organ is the basis of seed growth, and the development of rice floral organ directly affects the yield and quality. An open-hull mutant (oh) was found in the progenies of rive cultivar Yuanmeisimiao crossed with P704. The oh mutant displayed abnormal floral organ with unclosed lemma and palea, decreased seed setting rate and smaller seeds. Genetic analysis showed that the open-hull phenotype was controlled by a pair of recessive nuclear genes. Using bulked-segregant analysis (BSA) method, the open-hull gene was allocated in the interval 22806614~23000408 on rice chromosome 3, where the OsMADS1 gene (LOC_Os03g11614) is present. Through alignment with the homologous gene in the Shuhui498 reference genome, the nucleotide transversion (aspartic acid to tyrosine) at the 118th nucleotide of the first exon of OsMADS1 in oh was identified (designated OsMADS1oh). OsMADS1oh was cloned from oh and used to construct overexpression vector. The overexpression vector was introduced into the transformable material (ZH11) by Agrobacterium-mediated transformation. The lines over-expressing OsMADS1oh showed the open-hull phenotype. In addition, the RNA expression results of real-time quantitative PCR showed that the expression of MADS8 and YABB5 were regulated by OsMADS1. Collectively, this study approved the OsMADS1oh, as a new allelic variant of OsMADS1, and provided specific resources for elucidating the molecular mechanism of floral organ formation.

    Key words:rice (Oryza sativa L.);open-hull mutant;gene cloning
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毛兴学,郑晓钰,潘大建,等.水稻开颖突变体的遗传分析和基因克隆[J].植物遗传资源学报,2023,24(4):1114-1121.

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  • 收稿日期:2022-12-12
  • 最后修改日期:2023-03-27
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  • 在线发布日期: 2023-06-13
  • 出版日期: 2023-06-14
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