Potato cyst nematode （PCN） is an important quarantine disease and its infection can reduce the potato yield up to 80%. In recent years， the potato golden nematode （Globodera rostochiensis） was reported in several major producing areas in Southwest of China. In order to ensure the safety of potato production in China， it is urgent to identify germplasm resources with cyst resistance and breed for resistant varieties. Using the molecular markers N146， N195， 57R， TG689， Gro1-4-1 and Gpa2-2 from earlier studies， in this study 875 potato germplasm accessions preserved by Yunnan Academy of Agricultural Sciences were genotyped for resistance genes to potato cyst nematode. 211 accessions were detected with H1 molecular markers （N146，? N195， 57R and TG689） amplifying the resistance gene to potato cyst nematodes， of which 114 accessions contained four molecular markers at the same time. Seven accessions were identified carrying the resistance gene Gro1-4， while 237 resources were found with Gpa2-2 resistance gene Gpa2. Collectively， this study provided technical support and material basis for the breeding of potato cyst nematode resistance.