Drought is one of the most prominent factors that negatively affect the yield quantity and stability of wheat production. It is of great significance to select elite drought-resistant germplasm resources and cultivate drought-resistant varieties. In this study， the drought resistance of 180 wheat-Thinopyrum intermedium introgression lines was comprehensively evaluated at seed germination stage and adult plant stage. By measuring morphological indicators such as root length， biomass and yield-related traits， combined with principal component analysis， membership function analysis and correlation analysis， drought resistance at two growth stages was evaluated. The results showed that drought stress at two growth stages could result in significant decrease considering the drought-resistant related indicators. Shoot fresh weight， root fresh weight， shoot height and maximum root length decreased significantly at germination stage， and plant height and yield per plant decreased significantly at adult stage. The distributions of drought resistance coefficients of each indicator at different growth stages were significantly different. No significant correlation between the D value of comprehensive drought resistance evaluation at seed germination stage and adult stage was detected， but the D value at adult stage was significantly correlated with the drought resistance coefficients of yield per plant， the maximum root length and shoot dry weight at germination stage. The drought resistance of 180 derived lines was classified using the D value of comprehensive evaluation of drought resistance based on principal component analysis and membership function analysis. As a result， 24 lines at germination stage and 28 lines at adult stage were identified showing extremely drought resistant. Especially， 11 elite lines showing strong drought resistance at both germination and adult stages were obtained， which can be used as excellent germplasm resources for wheat drought-resistant breeding and genetic research.