This study investigated a special tomato inbred line 85-6 with the parthenocarpy and self-incompatibility. Through the observation of flower appearance， pollen viability and ovule development， 85-6 was found with normal flower structure， pollen activity and ovule development， thus ruling out the abortion of pistil or stamen. Based on the fertility variations of self-pollination， and artificially-assisted self-pollination and hybridization experiments at different temperature， 85-6 showed self-incompatibility under normal temperature conditions， and produced seedless fruit after self-pollination， as well as normal hybrid offspring whenever serving as male or female parents. Under temperature stress （daily average temperature <10 ℃ or >35 ℃） at flowering stage， 85-6 became complete pollination and fertilization to obtain seeded fruits， and its offspring seeds showed 29.75% seed germination rate. After emasculation at the bud stage， whenever under normal or lower temperature conditions， t85-6 was able to produce seedless fruit， thus indicating the characteristics of parthenocarpy. Collectively， the material of 85-6， as a newly-identified germplasm showing the parthenocarpy and self-incompatibility， provided a basis for the parthenocarpy and self-incompatibility breeding， and further deciphering the functional mechanisms.