1.湖南科技大学生命科学与健康学院/经济作物遗传改良与综合利用湖南省重点实验室，湘潭411201;2.中国农业科学院作物科学研究所，北京100081;3.西南大学农学与生物科技学院，重庆400700;4.长江大学农学院，湖北荆州434025
研究方向为荞麦分子机制研究，E-mail：910553160@qq.com
唐新科，研究方向为荞麦化感作用机制研究，E-mail：xinketang@126.com
国家重点研发计划（2019YFD1000700，2019YFD1000703）
- OUYANG Yinan 1,2
OUYANG Yinan
School of Life and Health Sciences， Hunan University of Science and Technology/ Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization， Xiangtan 411201;Institute of Crop Sciences， Chinese Academy of Agricultural Sciences， Beijing 100081
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在本站中查找 - LIU Yang 2,3
- PENG Jiashi 1
- CHEN Yuanyuan 2,4
- ZHAO Hui 2
- HE Yuting 1
- ZHAO Xinyu 1
- TANG Xinke 1
- ZHOU Meiliang 2
1.School of Life and Health Sciences， Hunan University of Science and Technology/ Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization， Xiangtan 411201;2.Institute of Crop Sciences， Chinese Academy of Agricultural Sciences， Beijing 100081;3.College of Agronomy and Biotechnology， Southwest University， Chongqing 400700;4.College of Agriculture， Yangtze University， Jingzhou 434025，Hubei
National Key R&D Program of China（2019YFD1000700，2019YFD1000703）
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前期苦荞转录组分析显示了一个介导黄酮类物质合成相关的黄酮合成酶基因FtFLS1。为进一步了解FtFLS1基因的结构、功能和基因多样性，本研究通过同源比对和保守序列分析筛选得到苦荞FLS基因家族共104个成员，其根据同源性和结构域分为10个亚族，FtFLS1属于DF8亚族。同时，启动子分析结果显示上游1500 bp启动子序列中有2个MeJA响应元件，据此，本研究分析了FtFLS1在苦荞不同器官中的表达量差异及其在MeJA不同处理时间下苦荞中的表达量差异，结果显示，FtFLS1在茎和叶中的表达相近且均要明显高于其在根中的表达，同时FtFLS1在苦荞中的表达也随着MeJA处理时间的增加而显著提高。为进一步验证FtFLS1的功能，本研究克隆了FtFLS1的CDS序列，以此构建FtFLS1的过表达苦荞毛状根株系并检测了它们的黄酮类物质含量，结果显示，相对于正常诱导的苦荞毛状根，FtFLS1的过表达毛状根明显积累了3类黄酮合成酶的下游产物：山奈酚、槲皮素和芦丁，而黄酮合成酶的底物二氢山奈酚和二氢槲皮素的含量明显降低。此外，本研究还分析了200份不同群体苦荞中FtFLS1基因的多样性，结果显示：北方群体、南方群体和喜马拉雅野生群体均拥有明显不同的FtFLS1基因型分布，其中北方群体和南方群体呈现明显的分化，研究结果为探索FtFLS1介导的黄酮类物质合成以及了解荞麦驯化过程提供了思路和参考。
The transcriptome analysis of tartary buckwheat identified a flavonol synthase gene FtFLS1. In order to further understand its structure， function and diversity in tartary buckwheat genome， we identified 104 members of FLS gene family with 10 subgroups， in which FtFLS1 was found in DF8 subgroup. Promoter analysis revealed two MeJA response elements at the upstream of 1500 bp sequence. We analyzed the expression of FtFLS1 in different organs and its response to MeJA treatments. The transcriptional level of FtFLS1 in stems and leaves was comparable but higher in roots. Expression of FtFLS1 also increased significantly with the treatment of MeJA. We subsequently cloned the CDS sequence of FtFLS1， and then generated FtFLS1 over-expressed hairy root lines of tartary buckwheat and detected their flavonoid content. Over-expression transformants over accumulated the downstream products of FLS， which including kaempferol， quercetin and rutin， while the contents of dihydrokaempferol and dihydroquercetin， the substrates of brass synthetase， decreased significantly. Furthermore， we analyzed the diversity of FtFLS1 gene in different populations of tartary buckwheat， and found that Northern landraces， Southwestern landraces and Himalayan wild accessions present obvious differentiation. The results are helpful for understanding the FtFLS1-mediated synthesis of flavonoids and the domestication process of buckwheat.
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欧阳屹南,刘洋,彭佳师,等.苦荞
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- 收稿日期:2023-02-26
- 最后修改日期:2023-03-10
- 在线发布日期: 2023-08-30
- 出版日期: 2023-08-30
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