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首页 > 过刊浏览>2023年第24卷第6期 >1626-1638. DOI:10.13430/j.cnki.jpgr.20230324002
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基于HS-SPME-GC-MS测定5种蔷薇属植物花瓣挥发性成分
作者:
作者单位:

中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室/农业农村部花卉生物学与种质创制重点实验室(北方)，北京 100081

作者简介:

研究方向为玫瑰花色花香代谢研究，E-mail ：793670339@qq.com

通讯作者:

杨树华，研究方向为花卉种质资源与遗传育种，E-mail： yangshuhua@caas.cn

基金项目:

山东省重点研发计划项目（2022LGZCQY009）；现代农业产业技术体系北京市创新团队（BAIC09-2023）；中央级公益性科研院所基本科研业务费专项（IVF-BRF2021016，IVF-BRF2022014）；国家重点研发计划项目（2018YFD1000401）


Detection of Volatile Organic Compounds in Five Rose Petals Based on Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry
Author:
  • WANG Hongqian

    WANG Hongqian

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • KOU Yaping

    KOU Yaping

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • YAO Chenyang

    YAO Chenyang

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • Meng Zhaoyang

    Meng Zhaoyang

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • WANG Jingjing

    WANG Jingjing

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • JIA Ruidong

    JIA Ruidong

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • ZHAO Xin

    ZHAO Xin

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • GE Hong

    GE Hong

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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  • YANG Shuhua

    YANG Shuhua

    Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops （North China）， Ministry of Agriculture and Rural Affairs ， Beijing 100081
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Affiliation:

Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops (North China)， Ministry of Agriculture and Rural Affairs ， Beijing 100081

Fund Project:

Foundation projects： Key R&D Program of Shandong Province， China （2022LGZCQY009）； Beijing Innovation Consortium of Agriculture Research System （BAIC09-2023）； Central Public-interest Scientific Institution Basal Research Fund （IVF-BRF2021016，IVF-BRF2022014）；National Key R&D Program of China （2018YFD1000401）

  • 摘要
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  • 参考文献 [29]
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    摘要:

    花瓣中挥发性成分对观赏植物的开发和利用起着重要作用。本研究采用顶空固相微萃取气质色谱联用技术，对5种蔷薇盛花期花瓣中挥发性成分进行检测分析，结果表明：5种蔷薇植物花瓣中共鉴定出91种挥发性成分，包含萜烯类、醇类、酯类、醛类、烷类、酚类、酮类、醚类和酸类9个类型。其中，百叶蔷薇共检测到45种挥发性成分，无特有成分，百叶蔷薇品种摩洛哥共检测到52种挥发性成分，5种特有成分；格拉斯共检测到40种挥发性成分，2种特有成分；菲茨詹姆斯共检测到57种挥发性成分，8种特有成分；大马士革蔷薇共检测出63种挥发性成分，11种特有成分。2-苯乙醇、香叶醇、香茅醇、乙酸香叶酯等是5种蔷薇花瓣中主要的挥发性成分，大马士革蔷薇花瓣的挥发性物质含量最多，达3622.68 ng/g，其次是摩洛哥、菲茨詹姆斯、格拉斯和百叶蔷薇，挥发性物质含量分别为2507.0 ng/g、2445.9 ng/g、1394.54 ng/g和883.38 ng/g。基于5种蔷薇花瓣中挥发性成分含量进行聚类分析发现，大马士革蔷薇与菲茨詹姆斯聚类关系较近，萜烯类挥发性成分种类和含量在5种蔷薇中差异较大。本研究为蔷薇属植物资源的开发和利用提供理论参考。

    Abstract:

    Fragrance volatile organic compounds play key role on exploitation and utilization in ornamental plants. In this study， headspace solid phase microextraction combined with gas chromatography mass spectrometry （HS-SPME-GC-MS） was used to determine and quantify the volatile organic compounds （VOCs） in five rose petals. Ninety-one VOCs were identified and classified into nine types： terpenes， alcohols， esters， aldehydes， alkanes， phenols， ketones， ethers， acids. Forty-five components were detected in R. centifolia； 52 components including five exclusive compositions were detected in R. ‘Morocco’； 40 compounds including two exclusive compositions were detected in R. ‘de Grasse’； 57 compounds including eight exclusive compositions were detected in R. ‘Duc de Fitzjames’； 63 compounds including 11 exclusive compositions were detected in R. × damascena. The compounds 2-Phenylethanol， Geraniol， β-Citronellol， and Geranyl acetate were predominant in five rose petals. R. × damascena had the highest content of VOCs， reaching 3622.68 ng/g， and the contents of VOCs in R. ‘Morocco’， R. ‘Duc de Fitzjames’， R. ‘de Grasse’， R. centifolia were 2507.07 ng/g， 2445.9 ng/g， 1394.54 ng/g， 883.38 ng/g， respectively. Heatmap cluster analysis and cluster analysis showed that R. × damascena was closely related to R.‘Duc de Fitzjames’， and the content of terpenes were significantly different in petals of five rose. Collectively， this study provided reference for future exploitation and utilization of Rosa genus resources.

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王洪乾,寇亚平,姚晨阳,等.基于HS-SPME-GC-MS测定5种蔷薇属植物花瓣挥发性成分[J].植物遗传资源学报,2023,24(6):1626-1638.

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  • 收稿日期:2023-03-24
  • 最后修改日期:2023-04-21
  • 在线发布日期: 2023-10-31
  • 出版日期: 2023-10-31
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