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首页 > 过刊浏览>2023年第24卷第6期 >1716-1724. DOI:10.13430/j.cnki.jpgr.20230426003
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一个水稻穗部突变体zhao1的遗传和连锁分析
作者:
作者单位:

1.安徽农业大学农学院，合肥230026;2.中国农业科学院作物科学研究所，北京100081

作者简介:

研究方向为水稻分子育种，E-mail：1441647747@qq.com；

通讯作者:

程治军，研究方向为水稻重要农艺性状基因克隆，E-mail：chengzhijun@caas.cn

基金项目:

科技创新项目（2022ZD04004）


Genetic and Linkage Analysis of a Rice Panicle Mutant zhao1
Author:
Affiliation:

1.College of Agronomy，Anhui Agricultural University，Hefei 230036;2.Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，Beijing 100081

Fund Project:

Foundation project： Technological Innovation Projects（2022ZD04004）

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    摘要:

    穗顶部退化型在生产中十分常见，它影响穗粒数、结实率和单株产量，需要在育种实践中尽量避免；而直立密穗型是一种能够适应密植栽培的穗型。分离和克隆这两个性状的相关基因对水稻的分子设计育种具有重要意义。本研究报道了一个水稻穗部突变体zhao1的研究结果。zhao1表现为密穗和穗顶部退化的双重表型，利用该材料与 IRAT129杂交，F2群体中分离出穗型和顶部退化两个独立遗传的性状。遗传分析表明，直立密穗性状为单基因显性性状，而穗顶部退化性状可能涉及不多于两对的基因。分别对这两个性状开展了连锁分析和图位克隆，发现密穗性状的候选基因为DEP1，其突变方式与dep1的突变方式相同。穗顶部退化性状的连锁和图位克隆结果将穗顶部退化候选基因确定在第3号染色体6.85~6.93 Mb区间内。该区间包含8个推测的基因，其中基因LOC_Os03g12790的表达量下调了27倍，是可能的候选基因，为最终克隆穗顶部退化候选基因奠定了基础。

    Abstract:

    Panicle apical abortion is often observed in rice production， which impairs the number of grains per panicle， the seed setting rate and the yield of single plant， and should be avoided in breeding practice. The dense and erect panicle is a plant type adaptable to dense planting. It is of importance to isolate the genes related to these two traits in molecular design breeding. Here， we present the research result of a rice panicle mutant zhao1， which displays the dense panicle and panicle apical abortion. Either dense panicle or panicle apical abortion was segregated independently in the F2 population derived from zhao1 crossed with IRAT129. Genetic analysis showed that the dense and erect panicle trait is controlled by a single dominant gene， while the panicle apical abortion trait may involve no more than two pairs of genes. Based on the linkage analysis and map-based cloning at both traits respectively， the gene DEP1 was found to be the candidate for the dense panicle trait in zhao1 which carries the identity mutation as the dep1. The linkage mapping and map-based cloning to the trait of panicle apical abortion finally delimited the candidate gene to the physical interval of 6.85-6.93 Mb on chromosome 3， where 8 putative genes are annotated. The gene LOC_Os03g12790 was observed with the most significant decrease on transcriptional profile， and served as the best candidate for the panicle apical abortion phenotype. Collectively， our results laid the foundation for the future cloning of the functional gene in rice.

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欧阳晨林,赵志超,邢欣欣,等.一个水稻穗部突变体zhao1的遗传和连锁分析[J].植物遗传资源学报,2023,24(6):1716-1724.

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  • 收稿日期:2023-04-26
  • 最后修改日期:2023-05-05
  • 在线发布日期: 2023-10-31
  • 出版日期: 2023-10-31
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