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首页 > 过刊浏览>2024年第25卷第3期 >462-471. DOI:10.13430/j.cnki.jpgr.20230818001
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新几内亚凤仙CCoAOMT和SAMS基因的克隆及表达分析
作者:
作者单位:

西南林业大学园林园艺学院/国家林业和草原局西南风景园林工程技术研究中心/云南省功能性花卉资源及 产业化技术工程研究中心/西南林业大学园林园艺花卉研发中心，昆明 650224

作者简介:

研究方向为园林植物遗传育种，E-mail：2098175323@qq.com

通讯作者:

黄美娟，研究方向为园林植物遗传育种，E-mail：xmhhq2001@163.com
黄海泉，研究方向为园林植物遗传育种，E-mail：haiquanl@163.com

基金项目:

国家自然科学基金（32060364，32060366）；云南省重大科技专项（202102AE090052）；园林植物遗传改良与高效繁育博士生导师团队项目（503210103）


Cloning and Expression Analysis of CCoAOMT and SAMS Genes in Impatiens Hawkeri
Author:
  • LI Fan

    LI Fan

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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  • ZHANG Xiaoli

    ZHANG Xiaoli

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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  • ZHAO Luqiu

    ZHAO Luqiu

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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  • SHI Wanlei

    SHI Wanlei

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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  • TAN Yi

    TAN Yi

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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  • HUANG Meijuan

    HUANG Meijuan

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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  • HUANG Haiquan

    HUANG Haiquan

    College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture （State Forestry and Grassland Administration）/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224
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Affiliation:

College of Landscape Architecture and Horticulture Sciences， Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration)/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/ Research and Development Center of Landscape Plants and Horticulture Flowers， Southwest Forestry University， Kunming 650224

Fund Project:

Foundation projects： The National Natural Science Foundation of China （32060364，32060366）；The Major Science and Technology Project of Yunnan Province （202102AE090052） ；The Doctoral Supervisor Team Project of Garden Plant Genetic Improvement and Efficient Breeding （503210103）

  • 摘要
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    摘要:

    通过对新几内亚凤仙（Impatiens hawkeri）指甲花醚合成相关基因CCoAOMT和SAMS的克隆及表达分析，以期为解析其合成途径提供一定的理论依据。基于新几内亚凤仙指甲花醚含量测定和转录组测序数据分析，克隆到1个CCoAOMT和2个SAMS基因，将其命名为IhCCoAOMT、IhSAMS1和IhSAMS2。3种处理（光培养、暗培养和前体物DHNA （1，4-dihydroxy-2-naphthoate）诱导）均能促进指甲花醚积累，且暗培养处理最佳。IhCCoAOMT的cDNA全长为729 bp，编码242 aa，其蛋白具有AdoMet_MTases超家族结构域；IhSAMS1和IhSAMS2的cDNA全长分别为1179 bp和1173 bp，分别编码393 aa和391 aa，其蛋白均具有S-AdoMet_synt超家族结构域。系统进化分析表明，IhCCoAOMT与喜马拉雅凤仙亲缘关系最近；IhSAMS1与IhSAMS2可能为旁系同源。qRT-PCR分析表明，3个基因在新几内亚凤仙的3个处理及4个时期中均有表达，其中IhCCoAOMT在暗培养及前体物DHNA诱导中表达量整体上调，且暗培养第60天时表达量最高；IhSAMS1和IhSAMS2在暗培养及DHNA诱导中表达量整体下调，但IhSAMS1在DHNA诱导中表达量较高，IhSAMS2在暗培养中表达量较高。综上所述，推测IhCCoAOMT在指甲花醚处理后期起主要作用，IhSAMS1和IhSAMS2在其早期起主要作用，且IhSAMS2作用更为显著。

    Abstract:

    Through cloning and analyzing the expression of the CCoAOMT and SAMS genes in Impatiens hawkeri， we provided a theoretical basis for understanding the biosynthesis of 2-methoxy-1，4-naphthoquinone （MNQ）. Based on the quantification of MNQ content and the transcriptome profile datasets in I. hawkeri ， one CCoAOMT and two SAMS genes of I. hawkeri were identified and cloned， named IhCCoAOMT， IhSAMS1 and IhSAMS2. Three treatments including light， dark and DHNA（1，4-dihydroxy-2-naphthoate） could promote the MNQ accumulation， with best performance under dark culture. The full-length coding sequence of IhCCoAOMT is 729 bp and encodes 242 aa containing the AdoMet_MTases superfamily domain. The CDS of IhSAMS1 and IhSAMS2 are 1179 bp and 1173 bp， which encode 393 aa and 391 aa， respectively. Their deduced proteins were predicted with the S-AdoMet_synt superfamily domain. Phylogenetic analysis showed that IhCCoAOMT was closely related to I. glandulifera. IhSAMS1 and IhSAMS2 of I. hawkeri may be paralogous genes. These genes were detected with transcripts at four culture periods under three treatments. The expression level of IhCCoAOMT increased in dark culture and DHNA treatment， and the highest expression was found on 60 days post dark culture. The expression levels of IhSAMS1 and IhSAMS2 were decreased in dark culture and DHNA treatment. The expression level of IhSAMS1 was higher than that in DHNA treatment， and a higher expression of IhSAMS2 was observed under dark culture treatment. Collectively， IhCCoAOMT was speculated with a key role at the late stage of MNQ treatment， while IhSAMS1 and IhSAMS2， especially for IhSAMS2， possibly played roles at the early stage.

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李凡,张晓丽,赵潞秋,等.新几内亚凤仙CCoAOMT和SAMS基因的克隆及表达分析[J].植物遗传资源学报,2024,25(3):462-471.

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  • 收稿日期:2023-08-18
  • 最后修改日期:2023-09-25
  • 在线发布日期: 2024-03-05
  • 出版日期: 2024-02-26
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