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首页 > 过刊浏览>2024年第25卷第3期 >356-372. DOI:10.13430/j.cnki.jpgr.20230827002
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286份甘蓝型油菜种质苗期耐盐碱性综合评价
作者:
作者单位:

1.江汉大学生命科学学院/湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心，武汉430056;2.华中农业大学植物科学技术学院/国家油菜工程技术研究中心，武汉 430070

作者简介:

主要从事特色植物资源开发与利用研究，E-mail：xty99100911@163.com

通讯作者:

曾长立，主要从事植物逆境生理、珍稀濒危植物保护与利用研究， E-mail：zengchangli@jhun.edu.cn
万何平，主要从事油菜耐盐碱遗传研究，E-mail：wanheping@jhun.edu.cn

基金项目:

生物育种专项（2022ZD04010）；国家自然科学基金（U22A20469 ）；国家重点研发计划项目（2016YFD0100202-25）


Comprehensive Evaluation on Saline-alkali Tolerance of 286 Brassica napus Germplasm at Seedling Stage
Author:
Affiliation:

1.College of Life Sciences， Jianghan University/Hubei Engineering Research Center for Conservation， Development and Utilization of Characteristic Biological Resources in Hanjiang River Basin， Wuhan 430056;2.College of Plant Science and Technology， Huazhong Agricultural University /National Oilseed Rape Engineering Technology Research Center， Wuhan 430070

Fund Project:

Foundation projects： Biological Breeding Special （2022ZD04010）；The National Natural Science Foundation of China （U22A20469）； The National Key Research and Development Program （2016YFD0100202-25）

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    摘要:

    对286 份甘蓝型油菜品系进行苗期耐盐碱性鉴定，通过水培试验，测定盐碱胁迫处理下叶片数、绿叶数、绿叶比、株高、根长、根重等指标，通过盐碱胁迫综合评价值（D值）、极端材料筛选分析、相关性、主成分、隶属函数、频数分析和逐步回归分析法，对不同基因型的油菜种质建立苗期耐盐碱性综合评价模型并筛选出适宜的评价鉴定指标。盐碱胁迫下，叶片数与株高呈负相关，两者的相关系数未达到显著性，其他性状之间均呈正相关并达到了显著或极显著水平。利用主成分分析法将 7个耐盐碱指标综合为4个主成分，可代表油菜耐盐碱性88.349%的原始数据信息量。依据4个主成分的相对重要性（权重）进行加权，得到不同基因型的耐盐碱性综合评价值（D值）。结合隶属函数分析和极端材料筛选分析，筛选出4份耐碱盐的甘蓝型油菜种质和4份盐碱敏感种质。逐步回归分析结果表明，在油菜苗期测定其绿叶数、绿叶比、地上重、根长和根重，通过回归方程估算其D值，可以初步判断甘蓝型油菜种质资源耐盐碱性。

    Abstract:

    The salt-alkali tolerance of 286 brassica rapeseed strains at the seedling stage was identified in this study. The indexes of leaves number， green leaves number， green leaves rate，shoot length， root length， and root weight under salt-alkali stress were determined through a hydroponics test. Various methods including comprehensive assessment value （D-value）， extreme material screening analysis， correlation analysis， principal component analysis， membership function analysis， frequency analysis and stepwise regression analysis were used. A comprehensive evaluation model for saline-alkali tolerance was established for different genotypes of rapeseed germplasm at the seedling stage and appropriate evaluation indexes were selected. Under saline-alkali stress conditions， leaves number showed a negative correlation with plant height but the correlation coefficient between them was not significant； however other traits showed positive correlations that reached a significant or extremely significant level. Principal component analysis was employed to synthesize 7 salt-alkali tolerance indexes into 4 principal components which could represent 88.349% of the original data information on rape′s salt-alkali tolerance. Based on the relative importance （weight） of these four principal components， a comprehensive evaluation value （D-value） for salt and alkali tolerance among different genotypes was obtained. Four alkali-salt-tolerant brassica napus germplasms and four saline-sensitive germplasms were screened using membership function analysis and extreme material screening analysis respectively. The results from stepwise regression analysis indicated that salinity tolerance in brassica napus germplasm resources could be determined by measuring the green leaves number， green leaves rate， shoot weight as well as root length and root weight at the seedling stage while estimating D-value using regression equations.

    参考文献
    [1] Yamaguchi T, Blumwald E. Developing salt-tolerant cropplants: Challenges and opportunities. Trends in Plant Science, 2005, 10(12): 615-620
    [2] 毛庆莲,王胜.国内盐碱地治理趋势探究浅析.湖北农业科学, 2020,59(S1): 302-306Mao Q L, Wang S. Analysis on the trend of saline-alkali land management in China. Hubei Agricultural Sciences, 2020, 59(S1): 302-306
    [3] 万林生,孙红芹,倪正斌,严国红,周汝琴.油菜盐油杂3号耐盐性试验及沿海滩涂全程机械化栽培技术.浙江农业科学, 2017,58(6):959-961Wan L S, Sun H Q, Ni Z B, Yan G H, Zhou R Q. Experimental study on salt tolerance of Salt-Youza-3 in rape and mechanized cultivation technology in coastal shoals. Zhejiang Agricultural Sciences, 2017, 58(6): 959-961
    [4] 王雷, 郭岩, 杨淑华. 非生物胁迫与环境适应性育种的现状及对策. 中国科学: 生命科学, 2021, 51(10): 1424-1434Wang L, Guo Y, Yang S H. Current situation and countermeasures of abiotic stress and environmental adaptive breeding. Scientia Sinica Vitae, 2021, 51(10): 1424-1434
    [5] 万何平, 张浩, 余忆, 陈敬东, 曾长立, 赵伦, 文静, 沈金雄, 傅廷栋.油菜耐盐碱研究与应用. 中国农业科技导报, 2022, 24(12): 59-67Wan H P, Zhang H, Yu Y, Chen J D, Zeng C L, Zhao L, Wen J, Shen J X, Fu T D. Research and application of salt-alkali tolerance in rapeseed. Journal of Agricultural Science and Technology, 2022, 24(12): 59-67
    [6] 马波. 氮肥、密度对寒地超级稻‘龙粳31’产量的互作效应研究. 中国农学通报, 2018, 34(6): 8-13Ma B. Study on interaction effect of nitrogen fertilizer and density on yield of super rice 'Longjing 31' in cold region. Chinese Agricultural Science Bulletin, 2018, 34(6): 8-13
    [7] 李霞, 曹昆, 阎丽娜, 王超, 孙志伟, 周月兰. 盐碱胁迫对不同水稻材料苗期生长特性的影响. 中国农学通报, 2008, 170(8): 252-256Li X, Cao K, Yan L N, Wang C, Sun Z W, Zhou Y L. Effects of saline-alkali stress on growth characteristics of different rice materials at seedling stage. Chinese Agricultural Science Bulletin, 2008, 170(8): 252-256
    [8] 吴鹏博, 李立军, 张艳丽. 油菜苗期耐盐碱性综合评价与根际土壤有机酸含量比较. 作物杂志, 2022(1): 110-115Wu P B, Li L J, Zhang Y L. Comprehensive evaluation of saline-alkali tolerance and comparison of rhizosphere soil organic acid content at rapeseed seedling stage. Crops, 2022(1): 110-115
    [9] 李琳, 于崧, 蒋永超, 张婷婷, 邹春雷, 金珊珊, 郭建华,梁海芸,段君君,于立河. 芸豆苗期耐盐碱性鉴定及品种筛选研究. 植物生理学报, 2016, 52(1): 62-72Li L, Yu S, Jiang Y C, Zhang T T, Zou C L, Jin S S, Guo J H, Liang H Y, Duan J J, Yu L H. Identification of saline-alkali tolerance and variety screening of kidney bean at seedling stage. Journal of Plant Physiology, 2016, 52(1): 62-72
    [10] 张林, 杨剑飞, 于立伟, 于晶, 王军军, 王振华, 刘显军. 玉米苗期耐盐碱鉴定体系优化及50份美国自交系耐盐碱性鉴定. 种子, 2016, 35(5): 94-98Zhang L, Yang J F, Yu L W, Yu J, Wang J J, Wang Z H, Liu X J. Optimization of salt-alkali tolerance identification system for maize seedlings and identification of salt-alkali tolerance of 50 American inbred lines. Seed, 2016, 35(5): 94-98
    [11] 王晓春, 杨天辉, 王川, 杨炜迪, 高婷. 混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响. 中国农学通报, 2022, 38(19): 139-145Wang X C, Yang T H, Wang C, Yang W D, Gao T. Effects of mixed salt and alkali stress on growth and physiological indexes of alfalfa. Chinese Agricultural Science Bulletin, 2022, 38(19): 139-145
    [12] 赵俊香, 任翠梅, 吴凤芝, 刘守伟, 王殿奎. 16份菊芋种质苗期耐盐碱性筛选与综合鉴定. 中国生态农业学报, 2015, 23(5): 620-626Zhao J X, Ren C M, Wu F Z, Liu S W, Wang D K. Screening and comprehensive identification of saline-alkali tolerance of 16 Jerusalem artichoke germplasm at seedling stage. Chinese Journal of Eco-Agriculture, 2015, 23(5): 620-626
    [13] Gomesfilho E, Lima C R, Costa J H, Silva A C, Lima M D, Lacerda C F. Cowpea ribonuclease: Properties and effect of NaCl-salinity on its activation during seed germination and seedling establishment. Plant Cell Reports, 2008, 27(1): 147-157
    [14] 杨少辉, 季静, 王罡, 宋英今. 盐胁迫对植物影响的研究进展. 分子植物育种, 2006(S1):139-142Yang S H, Ji J, Wang G, Song Y J. Research progress on effects of salt stress on plants. Molecular Breeding, 2006(S1):139-142
    [15] Wang Y, Nii N. Changes in chlorophyll, ribulose biphosphate carboxylase-oxygenase, glycine betaine content, photosynthesis and transpiration in Amaranthus tricolor leaves during salt stress. The Journal of Horticultural Science and Biotechnology, 2000, (75): 623-627
    [16] 沈金雄, 傅廷栋.我国油菜生产,改良与食用油供给安全. 中国农业科技导报, 2011, 13 (1): 1-8Shen J X, Fu T D. Rape production improves and edible oil supply safety. Journal of Agricultural Science and Technology, 2011, 13(1): 1-8
    [17] 王汉中, 殷艳. 我国油料产业形势分析与发展对策建议. 中国油料作物学报, 2014, 36 (3): 414-421Wang H Z, Yin Y. Situation analysis and development countermeasure of oil industry of our country. Chinese Journal of Oil Crops Sciences, 2014, 36(3): 414-421
    [18] Banaei-asl F, Bandehagh A, Uliaei E D, Farajzaden D, Sakata K, Mustafa G, Komatsu S. Proteomic analysis of canola root inoculated with bacteria under salt stress. Journal of Proteomics, 2015, 124: 88-111
    [19] 汪波,文静,张凤华,李立军,来永才,任长忠,鲁剑巍,沈金雄,郭亮,周广生,傅廷栋.耐盐碱油菜品种选育及修复利用盐碱地研究进展.科技导报, 2021, 39(23): 59-64Wang B, Wen J, Zhang F H, Li L J, Lai Y C, Ren C Z, Lu J W, Shen J X, Guo L, Zhou G S, Fu T D. Research progress on breeding and restoration of saline-alkali soil for saline-alkali resistant rapeseed varieties. Science and Technology Review, 2021, 39(23): 59-64
    [20] 万何平, 戴希刚, 陈敬东,戢强强,曾长立. 甘蓝型油菜对盐胁迫的响应及耐盐相关性状QTL研究进展. 中国油料作物学报, 2020, 42 (4): 536-544Wan H P, Dai X G, Chen J D, Ji Q Q, Zeng C L. Research progress on salt stress effect on Brassica napus and QTL reaserch of salt tolerance related traits. Chinese Journal of Oil Crops Sciences, 2020, 42(4): 536-544
    [21] 张培通, 张萼, 郭文琦, 陈建平, 李春宏, 王永慧, 胡茂龙. 油菜宁杂21号在江苏沿海滩涂盐碱地的种植表现及高产栽培技术要点. 江苏农业科学, 2014, 42(6): 84-85Zhang P T, Zhang E, Guo W Q, Chen J P, Li C H, Wang Y H, Hu M L. Planting performance of rapeseed Ningza 21 in saline-alkali land of Jiangsu coastal flat and key points of high-yield cultivation techniques. Jiangsu Agricultural Sciences, 2014, 42(6): 84-85
    [22] 杨洋, 王亚娟, 阴法庭, 张凤华. 盐碱胁迫对油菜苗期生理及光合特性的影响. 北方园艺, 2020, 462(15): 1-8Yang Y, Wang Y J, Yin F T, Zhang F H. Effects of saline-alkali stress on physiological and photosynthetic characteristics of rape seedlings. Northern Horticulture, 2020, 462(15): 1-8
    [23] 李班, 吕莹, 杨明煊, 宋婷, 于放, 刘志文. 盐碱胁迫对甘蓝型油菜生理及分子机制的影响. 华北农学报, 2022, 37(3): 86-93Li B, Lv Y, Yang M X, Song T, Yu F, Liu Z W. Effects of saline-alkali stress on physiological and molecular mechanisms of Brassica napus L.. Journal of North China Agronomy, 2022, 37(3): 86-93
    [24] 李萍, 燕佳琦, 张鹤, 张燕, 陶顺仙, 张琪. 146份甘蓝型油菜种质芽期耐盐性筛选及评价. 西北农业学报, 2021, 30(6): 848-859Li P, Yan J Q, Zhang H, Zhang Y, Tao S X, Zhang Q. Screening and evaluation of salt tolerance in 146 Brassica napus germplasm at bud stage. Northwest Agricultural Journal, 2021, 30(6): 848-859
    [25] 万何平, 余忆, 陈敬东, 鲁金春子, 冉景鸿, 戴希刚, 文静, 傅廷栋, 沈金雄, 曾长立. 甘蓝型油菜耐盐碱性快速鉴定方法与应用. 中国油料作物学报, 2023, 45(4):776-784Wan H P, Yu Y, Chen J D, Lu J C Z, Ran J H, Dai X G, Wen J, Fu T D, Shen J X, Zeng C L. Rapid identification method and application of salt-alkali tolerance in Brassica napus. Chinese Journal of Oil Crops, 2023, 45(4): 776-784
    [26] 朱宗河, 郑文寅, 张学昆. 甘蓝型油菜耐旱相关性状的主成分分析及综合评价. 中国农业科学, 2011, 44(9): 1775-1787Zhu Z H, Zheng W Y, Zhang X K. Principal component analysis and comprehensive evaluation on morphological and agronomic traits of drought tolerance in rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 2011, 44(9): 1775-1787
    [27] 李丰先, 周宇飞, 王艺陶, 孙璐, 白薇, 闫彤, 许文娟, 黄瑞冬. 高粱品种萌发期耐碱性筛选与综合鉴定. 中国农业科学, 2013, 46(9): 1762-1771Li F X, Zhou Y F, Wan Y T, Sun L, Bai W, Yan T, Xu W J, Huang R D. Screening and comprehensive identification of alkaline resistance of sorghum varieties at germination stage.Scientia Agricultura Sinica, 2013, 46(9): 1762-1771
    [28] 杨阳. 盐碱地中国潜在的耕地资源. 中国农村科技, 2018 (11):8-13Yang Y. Saline-alkali land is the potential cultivated land resources in China. Chinese Rural Science and Technology, 2018 (11): 8-13
    [29] 刘东洋, 徐接亮, 张凤华. 不同油菜品种对盐碱土壤理化性质与微生物多样性的影响. 新疆农业科学, 2019, 56(2): 246-257.Liu D Y, Xu J L, Zhang F H. Effects of different rapeseed varieties on physicochemical properties and microbial diversity of saline-alkali soil. Xinjiang Agricultural Sciences, 2019, 56(2): 246-257
    [30] 万林生, 孙红芹, 倪正斌, 严国红, 周汝琴. 油菜盐油杂3号耐盐性试验及沿海滩涂全程机械化栽培技术. 浙江农业科学, 2017, 58(6): 959-961Wan L S, Sun H Q, Ni Z B, Yan G H, Zhou R Q. Salt-tolerance test of rape Salt-oil zaza No.3 and mechanized cultivation technology of coastal shoals. Zhejiang Agricultural Sciences, 2017, 58(6): 959-961
    [31] 高建明,夏卜贤,袁庆华,罗峰,韩芸,桂枝,裴忠有,孙守钧 . 高粱种质材料幼苗期耐盐碱性评价. 应用生态学报,2012,23(5): 1303-1310Gao J M, Xia B X,Yuan Q H, Luo F, Han Y, Gui Z, Pei Z Y, Sun S J. Sorghum seedling period and salt-tolerant germplasm materials evaluation. Chinese Journal of Applied Ecology, 2012, 23(5): 1303-1310
    [32] 张春宵,袁英,刘文国,李文华,王丹,李万军,李晓辉 . 玉米杂交种苗期耐盐碱筛选与大田鉴定的比较分析. 玉米科学,2010,18 (5) : 14-18Zhang C X, Yuan Y, Liu W G, Li W H, Wang D, Li W J, Li X H. Comparative analysis of salt-alkali tolerance screening and field identification of maize hybrids at seedling stage. Maize Science, 2010, 18 (5) : 14-18
    [33] 万何平. 甘蓝型油菜苗期耐盐相关性状的全基因组关联分析. 武汉: 华中农业大学, 2017Wan H P. Genome-wide association analysis of salt-tolerant traits in Brassica napus seedlings. Wuhan: Huazhong Agricultural University, 2017
    [34] Wani A S, Ahmad A, Hayat S, Fariduddin Q. Salt-induced modulation in growth, photosynthesis and antioxidant system in two varieties of Brassica juncea. Saudi Journal of Biological Sciences, 2013, 20(2): 183-193
    [35] Hayat S, Maheshwari P, Wani A S, Irfan M, Alyemenl M N, Ahmad A. Comparative effect of 28 homobrassinolide and salicylic acid in the amelioration of NaCl stress in Brassica juncea L. Plant Physiology and Biochemistry, 2012, 53: 61-68
    [36] Kaur H, Sirhindi G, Bhardwaj R, Alyemeni M N, Siddique K H, Ahmad P. 28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt-and temperature-induced oxidative stress in Brassica juncea. Scientific Reports, 2018, 8(1): 1-13
    [37] 朱孔志, 吴明昊, 申玉香, 李洪山, 杨世才, 杜希宽, 丁世峰. 不同油菜品种在盐碱地的耐盐性鉴定及筛选. 浙江农业科学, 2018, 59(8): 1354-1356Zhu K Z, Wu M H, Shen Y X, Li H S, Yang S C, Du X K, Ding S F. Identification and screening of salt tolerance of different rape varieties in saline-alkali soil. Zhejiang Agricultural Science, 2018, 59(8): 1354-1356
    [38] 王军, 周美学, 许如根, 吕超, 黄祖六. 大麦耐湿性鉴定指标和评价方法研究. 中国农业科学, 2007, 40(10): 2145-2152Wang J, Zhou M X, Xu R G, Lv C, Huang Z L. Study on indexes and evaluation methods of moisture resistance of barley.Scientia Agricultura Sinica, 2007, 40(10): 2145-2152
    [39] 祁旭升, 王兴荣, 许军, 张建平, 米君. 胡麻种质资源成株期抗旱性评价. 中国农业科学, 2010, 43(15): 3076-3087Qi X S, Wang X R, Xu J, Zhang J P, Mi J. Evaluation of drought resistance of flax germplasm resources at adult stage.Scientia Agricultura Sinica, 2010, 43(15): 3076-3087
    [40] 李丰先, 罗磊, 李亚杰, 姚彦红, 范奕, 李德明. 基于PCA和隶属函数法分析的马铃薯创新种质抗旱性鉴定与分类.干旱区资源与环境, 2022, 36(11): 141-147Li F X, Luo L, Li Y J, Yao Y H, Fan Y, Li D M. Identification and classification of drought resistance of potato germplasm based on PCA and membership function analysis. Arid Area Resources and Environment, 2022, 36(11): 141-147
    [41] 熊洁, 邹小云, 陈伦林, 李书宇, 邹晓芬, 宋来强. 油菜苗期耐铝基因型筛选和鉴定指标的研究. 中国农业科学, 2015, 48(16): 3112-3120Xiong J, Zou X Y, Chen L L, Li S Y, Zou X F, Song L Q. Study on screening and identification indexes of Aluminum tolerance genotypes in rape seedling stage.Scientia Agricultura Sinica, 2015, 48(16): 3112-3120
    [42] 刘芯欣,侯云龙,杜楠琳,李健琳,陈亮,崔正果,姬文秀,邱红梅,王跃强.大豆耐低氮资源的苗期鉴定与筛选.植物遗传资源学报, 2023, 24(2): 408-418Liu X X, Hou Y L, Du N L, Li J L, Chen L, Cui Z G, Ji W X,Qiu H M, Wang Y Q. Identification and screening of low nitrogen tolerance resources of soybean at seedling stage. Journal of Plant Genetic Resources, 2023, 24(2): 408-418
    [43] 霍宏亮,王超,杨祥,曹玉芬,田路明,董星光,张莹,齐丹,徐家玉,刘超. 杜梨对盐碱胁迫的生理响应及耐盐碱性评价. 植物遗传资源学报, 2022, 23(2): 480-492Huo H L, Wang C, Yang X, Cao Y F, Tian L M, Dong X G, Zhang Y, Qi D, Xu J Y, Liu C. Physiological response and salt-alkali tolerance evaluation of Fructus pyri to salt-alkali stress. Journal of Plant Genetic Resources, 2022, 23(2): 480-492
    [44] Nielsen D C, Nelson N O. Black bean sensitivity to water stress at various growth stages. Crop Science, 1998, 38(2): 422-427
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薛天源,鲁金春子,何思晓,等.286份甘蓝型油菜种质苗期耐盐碱性综合评价[J].植物遗传资源学报,2024,25(3):356-372.

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植物遗传资源学报 ® 2025 版权所有
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