芥菜型油菜茎秆抗倒伏相关性状的组织观察与QTL初定位
作者:
作者单位:

贵州大学农学院,贵阳550025

作者简介:

主要从事油菜育种与改良,E-mail:2694906114@qq.com

通讯作者:

田恩堂,主要从事油菜分子育种工作,E-mail: erictian121@163.com

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基金项目:

贵州省科技支撑计划项目(黔科合支撑[2022]重点031);国家自然科学基金(32160483,32360497);贵州省粮油作物分子育种重点实验室项目(黔科合中引地[2023]008)


Microstructure Observation and QTL Mapping of Traits Related to Stalk Lodging Resistance in Brassica juncea
Author:
Affiliation:

College of Agriculture, Guizhou University, Guiyang 550025

Fund Project:

Foundation projects: Guizhou Provincial Science and Technology Support Program Project (Qiankehe Support [2022] Key 031);National Natural Science Foundation of China (32160483,32360497);Guizhou Provincial Key Laboratory for Molecular Breeding of Grain and Oil Crops Project (Qiankehe Zhongyindi [2023] 008)

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    摘要:

    倒伏是严重降低油菜产量、品质和影响机械化生产的重要影响因素之一。培育和应用抗倒伏性强的油菜品种是实现油菜机械化生产和高产的重要措施,而提高抗倒伏能力的重点是提高茎秆强度。因此,本研究以包含197个芥菜型油菜株系的重组自交系(RILs,recombinant inbred lines )群体为研究材料,2023年分别在贵阳和贵定两个环境条件下测定了该群体的茎粗、茎秆鲜干比、茎秆充实度、茎秆密度、茎秆抗折力和茎秆抗折强度等6个性状,6个性状均表现出较大的变异,变异系数为14.29%~41.35%,且符合正态分布。相关性分析显示,两个环境条件下,茎秆充实度与茎秆密度、抗折强度二者之间均呈极显著正相关。进一步对茎秆抗折强度表现为高中低的3类材料进行茎秆微观结构观察,相比较于低抗倒材料,高抗倒材料的皮层更厚、维管束个数更多、维管束排列更紧密与维管束面积占比更大。QTL初定位检测到4个QTL与茎秆茎粗有关;2个QTL与茎秆鲜干比有关;2个QTL与茎秆充实度有关;2个QTL与茎秆密度有关;4个QTL与茎秆抗折力有关,可解释的表型变异为4.1%~5.1%;9个QTL与茎秆抗折强度有关,可解释的表型变异为7.5%~11.0%。本研究的开展可为后续芥菜型油菜抗倒伏相关基因的克隆提供基础数据信息,并对油菜抗倒伏育种有一定参考价值。

    Abstract:

    Lodging is an important influencing factor that seriously reduces the yield and quality of rapeseed and is undesirable in mechanized production. Cultivation of rapeseed varieties with strong lodging resistance is an important measure to achieve mechanized production and high yield performance, while the focus of improving lodging resistance is to improve stem strength. Therefore, a recombinant inbred lines (RILs) population containing 197 mustard rapeseed lines was used in this paper. Six characters including stem diameter, fresh-dry ratio, stem filling degree, stem density, stem bending resistance and stem bending strength were investigated under two environmental conditions in Guiyang and Guiding in 2023, respectively. All 6 personality traits showed significant variation, ranging from 14.29%-41.35%, and were in line with a normal distribution. Correlation analysis under two environmental conditions shows a highly significant positive correlation in stem plumpness if compared to stem density and bending strength. Further observation was conducted on the microstructure of the stems of three types of materials showing high, medium, and low bending resistance. Compared to low resistance materials, high resistance materials have thicker cortex, more vascular bundles, tighter arrangement of vascular bundle, and a larger proportion of vascular bundle area. In addition, 4 QTL for stem thickness, 2 QTL for stem fresh-dry ratio, 2 QTL for stem plumpness, and 2 QTL for stem density; 4 QTL for stem bending resistance explained the phenotypic variation of 4.1%-5.1%; 9 QTL for stem strength explained the phenotypic variation of 7.5%-11.0%. The results of this study provided basic data information for future cloning of lodging resistance related genes in mustard rapeseed, becoming valuable for lodging resistance breeding in rapeseed.

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王倩,杨旭,张金泽,等.芥菜型油菜茎秆抗倒伏相关性状的组织观察与QTL初定位[J].植物遗传资源学报,2024,25(3):431-439.

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  • 收稿日期:2023-09-04
  • 最后修改日期:2023-10-11
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  • 在线发布日期: 2024-03-05
  • 出版日期: 2024-02-26
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