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首页 > 过刊浏览>2024年第25卷第4期 >509-521. DOI:10.13430/j.cnki.jpgr.20230905002
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94份小麦种质Puroindoline和HMW-GS分子检测与品质分析
作者:
作者单位:

1.江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室，扬州 225007;2.玉米生物育种全国重点实验室，沈阳 110164

作者简介:

研究方向为作物遗传育种，E-mail ：zhangxiao820218@163.com

通讯作者:

陆成彬，研究方向为作物遗传育种，E-mail ：lucb123@126.com

基金项目:

江苏省农业科技自主创新资金（CX（21）3102）； 国家自然科学基金项目（32071999）； 扬州市重点研发计划（YZ2022049）


Molecular Detection of Puroindoline and HMW-GS and Quality Traits Analysis in 94 Wheat Germplasms
Author:
Affiliation:

1.Institute of Agricultural Sciences for Lixiahe region in Jiangsu /Key Laboratory of Wheat Biology and Genetic Improvement for Low＆Middle Yangtze Valley， Ministry of Agriculture and Rural Affairs， Yangzhou 225007;2.National Key Laboratory of Maize Biological Breeding， Shenyang 110164

Fund Project:

Foundation projects： Independent Innovation of Agricultural Science and Technology of Jiangsu Province （CX（21）3102）； National Natural Science Foundation of China（32071999）； Key Research and Development Program of Yangzhou City（YZ2022049）

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    摘要:

    籽粒硬度和高分子量谷蛋白亚基（HMW-GS）对小麦品质起决定作用，为发掘和利用硬度Puroindoline基因和HMW-GS优异等位变异，提升长江中下游麦区中强筋小麦品质，对长江中下游麦区推广品种以及其他麦区优质推广品种和地方品种共计94份材料进行分子检测和品质分析。结果表明，硬度变幅7.21~72.91，软质类型42份、占44.68%，硬质类型42份、占44.68%，混合类型10份、占10.64%。硬度突变基因型共有5种，包括Pina-D1b/Pinb-D1a、Pina-D1r/Pinb-D1a、Pina-D1s/Pinb-D1a、Pina-D1a/Pinb-D1b和Pina-D1a/Pinb-D1p，数量分别为8份、3份、1份、29份和9份，籽粒硬度表现依次为Pina-D1r/Pinb-D1a＞Pina-D1s/Pinb-D1a＞Pina-D1b/Pinb-D1a＞Pina-D1a/Pinb-D1p＞Pina-D1a/Pinb-D1b。HMW-GS分析表明，Glu-A1位点1和Null亚基材料比例分别为53.33%和45.56%，此外有1G330E亚基材料1份；Glu-B1位点7+8和7+9亚基材料比例分别为47.78%和46.67%，此外有14+15亚基材料3份、7OE+8*亚基材料1份、6+8亚基材料1份；Glu-D1位点2+12和5+10亚基材料比例分别为61.11%和38.89%。在微量SDS沉淀值上，Glu-A1位点的1和Null亚基、Glu-B1位点的7+8和7+9亚基无显著差异，Glu-D1位点5+10亚基极显著大于2+12亚基。硬度和SDS沉淀值呈极显著正相关，硬度对SDS沉淀值影响大于HMW-GS。本研究对小麦种质Pin和HMW-GS基因型和品质进行了分析，为小麦品质遗传改良尤其是中强筋小麦品质改良提供了参考。

    Abstract:

    Grain hardness and HMW-GS play a key role in wheat quality. In order to explore and utilize favorable allelic variants of wheat hardness Puroindoline gene and HMW-GS to improve the quality of medium-strong gluten， molecular detection of both genes and quality traits were analyzed in 94 wheat germplasms which mainly include varieties in the Low and Middle Yangtze River Valley Wheat Zone， as well as high quality wheat varieties or landraces in other wheat zones. The results showed that the grain hardness was ranged from 7.21 to 72.91. There were 42 soft wheat accounting for 44.68%， 42 hard wheat accounting for 44.68%， and 10 mixed wheat accounting for 10.64%. Five alleles at the Puroindoline locus were detected， including Pina-D1b/Pinb-D1a，Pina-D1r/Pinb-D1a，Pina-D1s/Pinb-D1a，Pina-D1a/Pinb-D1b and Pina-D1a/Pinb-D1p， with quantity of 8， 3， 1， 29 and 9 respectively. The hardness of different Puroindoline alleles ranked in the order： Pina-D1r/Pinb-D1a＞Pina-D1s/Pinb-D1a＞Pina-D1b/Pinb-D1a＞Pina-D1a/Pinb-D1p＞Pina-D1a/Pinb-D1b. HMW-GS subunits 1 and Null accounted for 53.33% and 45.56% respectively at Glu-A1 locus and one with 1G330E. 7+8 and 7+9 subunits accounted for 47.78% and 46.67% respectively at Glu-B1 locus. Furthermore， there were three wheat varieties carrying 14+15 subunits， one carrying 7OE+8* and one carrying 6+8 at Glu-B1 locus. 2+12 and 5+10 subunits accounted for 61.11% and 38.89% respectively. For SDS sedimentation value， there were no significant difference between 1 and Null subunits at Glu-A1 locus and 7+8 and 7+9 at Glu-B1 locus. The SDS sedimentation value of 5+10 subunits was higher than 2+12 subunits at Glu-D1 locus. A significant positive correlation between hardness and SDS sedimentation value was detected. The SDS sedimentation value was contributed by grain hardness， greater than that of HMW-GS. The genotypes of Puroindoline and HMW-GS and phenotypes of wheat germplasms were analyzed in this study， which provided a reference for the genetic improvement of wheat quality， especially for medium-strong gluten wheat.

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张晓,江伟,高德荣,等.94份小麦种质Puroindoline和HMW-GS分子检测与品质分析[J].植物遗传资源学报,2024,25(4):509-521.

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  • 收稿日期:2023-09-05
  • 最后修改日期:2023-11-20
  • 在线发布日期: 2024-04-29
  • 出版日期: 2024-04-16
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