62份葡萄种质资源果实香气物质遗传多样性分析
作者:
作者单位:

1.山西农业大学园艺学院,晋中 030800;2.山西农业大学果树研究所/果树种质创制和利用山西省重点实验室,太原 030031

作者简介:

的研究方向为葡萄种质资源评价及育种,E-mail:2261100501@qq.com

通讯作者:

赵旗峰,研究方向为葡萄种质资源评价及育种,E-mail:gssqfzhao@163.com
荀志丽,研究方向为葡萄种质资源评价及育种,E-mail:xzlgss@163.com

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基金项目:

农业农村部作物种质资源精准鉴定项目(19221993);农业农村部种质资源收集保存项目(19221876);山西省科技创新重点人才团队(202204051002037);山西农业大学生物育种工程项目(YZGC032)


Genetic Diversity Analysis of Aroma Substances in 62 Grape Germplasm Resources
Author:
Affiliation:

1.College of Horticulture, Agricultural University of Shanxi, Jinzhong 030800;2.Institute of Fruit Trees, Shanxi Agricultural University/Shanxi Key Laboratory of Fruit Germplasm Creation and Utilization, Taiyuan 030031

Fund Project:

Foundation projects: Precision Identification Project of Crop Germplasm Resources,Ministry of Agriculture and Rural Affairs (19221993); Ministry of Agriculture and Rural Affairs Germplasm Resource Collection and Preservation Project (19221876); Shanxi Provincial Key Talent Team for Science and Technology Innovation (202204051002037);Biological Breeding Engineering Project of Shanxi Agricultural University (YZGC032)

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    摘要:

    为了探究葡萄种质资源果实香气成分的遗传多样性,以62份葡萄种质资源作为试验材料,采用顶空固相微萃取结合气相色谱-质谱联用技术检测样品中的挥发性物质,进行显著性分析。鉴定葡萄种质资源幼叶上表面颜色、幼叶上表面光泽、幼叶花青素着色和成熟果肉香味等8个农艺性状,将其与挥发性物质进行相关性分析,并进一步通过非度量多维尺度分析(NMDS,non-metric multidimensional scaling,stress<0.2)和正交偏最小二乘-判别分析(OPLS-DA,orthogonal partial least squares-discriminant analysis)筛选出特征挥发性物质。结果表明,在62份葡萄种质资源中共检测到7类99种挥发性物质,主要是醇类、酯类和萜烯类,含量分别为13.83 mg/L、13.53 mg/L、8.99 mg/L。各类挥发性物质之间存在显著的差异。8个农艺性状与香气物质相关性分析结果显示,幼叶上表面颜色、上表面光泽、花青素着色、果皮颜色、果肉香味与萜烯类、酯类及酮类具有显著相关性。非度量多维尺度分析可以明显区分幼叶花青素着色极弱的种质和幼叶花青素着色中的种质。以变量投影重要性VIP(Variable importance in projection)>1,P<0.05为标准,筛选到17种主要的挥发性物质,其中正己酸乙酯的VIP值最高(4.07),萜烯类中橙花醇VIP值最高(2.07)。本研究结果可为改善浆果香气品质性状和葡萄种质资源的创新提供一定的理论参考。

    Abstract:

    To investigate the genetic diversity of aroma components in grape germplasm resources, 62 grape germplasm resources were used as experimental materials. Headspace solid-phase microextraction combined with gas chromatography-mass spectrometry was used to detect volatile compounds, and significance analysis of aroma compounds was conducted. 8 agronomic traits were identified, such as color of the upper side, sheen on upper side, anthocyanin colouration of young leaves, articular flavor of mature berries, and so on. Correlation analysis was carried between volatile compounds and 8 agronomic traits. The characteristic volatile substances compounds was screened out by non-metric multidimensional scale analysis (NMDS, stress<0.2) and orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that a total of 99 volatile substances in 7 categories were detected in 62 grape germplasm resources, mainly alcohols, esters and terpenes, with the contents of 13.83 mg/L, 13.53 mg/L and 8.99 mg/L, respectively. There are significant differences among various volatile substances. The results of correlation analysis between 8 agronomic traits and aroma substances showed that the color of the upper side,sheen on upper side, anthocyanin colouration of young leaves, color of skin and articular flavor of mature berries were significantly correlated with terpenes, esters and ketones. Non-metric multidimensional scaling analysis can clearly distinguish the germplasms with very weak anthocyanin coloring of young leaves and the germplasms with middle anthocyanin coloring of young leaves. Based on the variable importance in projection (VIP)>1, P<0.05 as the standard, 17 main volatile substances were screened, among which the VIP value of ethyl caproate was the highest (4.07), and the VIP value of nerol in terpenes was the highest (2.07). This study provides a theoretical reference for improving the aroma quality of berries and the innovation of grape germplasm resources.

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王环,许艺凡,张江妹,等.62份葡萄种质资源果实香气物质遗传多样性分析[J].植物遗传资源学报,2024,25(7):1092-1105.

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  • 收稿日期:2023-11-22
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  • 在线发布日期: 2024-07-04
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