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首页 > 过刊浏览>2024年第25卷第10期 >1726-1736. DOI:10.13430/j.cnki.jpgr.20240110001
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一粒系小麦遗传多样性分析及抗条锈菌CYR34鉴定
作者:
作者单位:

1.中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室，北京 100193;2.农业农村部国家植物保护甘谷观测实验站，甘肃甘谷 741200

作者简介:

研究方向为植物抗病性遗传，E-mail： xxw15621405359@163.com

通讯作者:

冯 晶，研究方向为植物抗病性遗传，E-mail： jingfeng@ippcaas.cn
蔺瑞明，研究方向为分子植物病理学，E-mail： linruiming@caas.cn

基金项目:

国家重点研发计划（2021YFD1401000）； 中国农业科学院科技创新工程


Genetic Diversity Analysis and Identification of Stripe Rust Resistance to CYR34 in Einkorn Wheat
Author:
Affiliation:

1.Institute of Plant Protection， Chinese Academy of Agricultural Sciences/State Key Laboratory for Biology of Plant Diseases and Insect Pests， Beijing 100193;2.National Agricultural Experimental Station for Plant Protection at Gangu， Ministry of Agriculture and Rural Affairs， Gangu 741200， Gansu

Fund Project:

Foundation projects： National Key R&D Program of China （2021YFD1401000）； Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences

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    摘要:

    一粒系小麦（Einkorn wheat， AA）作为小麦的基础物种，在形成普通小麦的过程中染色体组部分位点丢失，评价一粒系小麦的遗传多样性及对病害抗性的水平对普通小麦育种和遗传改良具有重要的理论意义和育种价值。本研究利用15对条带清晰、多态性高的SSR引物对170份一粒系小麦材料进行遗传多样性分析，并接种条锈菌流行生理小种CYR34进行抗病性评价。结果表明，SSR分析获得71个等位变异，引物平均多态性信息含量为0.6540；聚类分析和群体结构分析均将170份供试材料分为两个类群，两类群内的平均遗传距离分别为0.4732和0.5404；抗病性评价获得19份抗性较好的材料，其中免疫材料3份，近免疫材料2份，高抗1份，中抗13份，占供试材料的11.17 %；有3对SSR引物与一粒系小麦抗条锈病显著相关。综上所述，一粒系小麦存在较多的等位基因变异，含有优异的抗条锈病基因，具有提高小麦抗条锈病的育种潜力。

    Abstract:

    Einkorn wheat （AA）， as the basic species of wheat， has lost some loci of the A chromosome during the evolution of bread wheat. Evaluating the genetic diversity and disease resistance level of einkorn wheat has important theoretical significance and breeding value for common wheat breeding and genetic improvement. In our study， 15 pairs of SSR primers with clear bands and high polymorphism were used to analyze the genetic diversity of 170 einkorn wheat materials， and the current epidemic stripe rust physiological race CYR34 was used for disease resistance evaluation. The results showed that 71 alleles were obtained by SSR analysis， and the average polymorphism information content of primers was 0.6540. Cluster analysis and population structure analysis showed that tested materials were divided into two groups， and the average genetic distance within the two groups was 0.4732 and 0.5404， respectively. Through evaluation of disease resistance， 19 materials with better resistance were obtained， including 3 immunity， 2 near immunity， 1 high resistance and 13 medium resistance materials， accounting for 11.17% of the tested materials. Three pairs of primers had a correlation with the resistance of einkorn wheat to stripe rust. In summary， einkorn wheat contains abundant allelic variations and stripe rust resistance genes， which has the breeding potential to improve wheat resistance to stripe rust.

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徐晓伟,冯晶,王凤涛,等.一粒系小麦遗传多样性分析及抗条锈菌CYR34鉴定[J].植物遗传资源学报,2024,25(10):1726-1736.

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  • 收稿日期:2024-01-10
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