甘蓝型油菜茎秆强度相关性状QTL分析
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青海大学农林科学院/青海大学省部共建三江源生态与高原农牧业国家重点实验室/国家油菜改良中心青海分中心/ 青海省春油菜遗传改良重点实验室/青海省春油菜工程技术研究中心,西宁 810016

作者简介:

研究方向为作物种质资源,E-mail:qhrapelab@126.com

通讯作者:

杜德志,研究方向为作物杂种优势利用,E-mail:qhurape@126.com

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基金项目:

青海省科技项目(2023-NK-145);国家现代农业产业技术体系(CARS-12);国家重点研发计划(2018YFD0100500)


QTL Mapping of Stalk Strength-Related Traits in Rapeseed (Brassica napus L.)
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Affiliation:

Academy of Agricultural and Forestry Sciences, Qinghai University/State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University/Qinghai Research Branch of the National Rapeseed Genetic Improvement Center/Key Laboratory of Spring Rapeseed Genetic Improvement of Qinghai Province/Qinghai Engineering Technology Research Center for Spring Rapeseed, Xining 810016

Fund Project:

Foundation projects: Science and Technology Project of Qinghai Province (2023-NK-145);China Agriculture Research System(CARS-12);China National Key R&D Program(2018YFD0100500)

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    摘要:

    倒伏影响作物产量和机械收获。茎秆强度是作物抗倒育种的重要目标。本研究以茎秆强度及其相关性状存在显著差异的两个甘蓝型油菜品种(系)G922和中双11号为亲本构建的DH群体为材料,对该群体茎秆强度相关性状进行QTL分析。结果表明:(1)中双11号和G922的杂种F1在茎直径、茎壁厚度和茎折断力上存在明显的正向中亲优势;茎折断力与茎直径、茎壁厚度、茎壁木质部厚度、茎壁穿透力均呈极显著正相关,茎壁穿透力与茎壁木质部厚度呈极显著正相关。(2)构建了包含1984个SNP标记、总长度为2592.64 cM的甘蓝型油菜遗传连锁图谱,在4个环境下共检测到90个茎秆强度及其相关性状QTL;其中有17个QTL能在多环境下重复检测到,包括6个茎直径QTL、5个茎秆强度QTL、2个茎壁厚度QTL和4个茎折断力QTL;茎直径主效QTL cqSD.C8-1在4个环境下能重复检测到,对表型变异的贡献率为14.67%;A2染色体上4个茎直径QTL、C6染色体上4个茎秆强度QTL分别组成QTL簇cqSD.A2cqSS.C6。(3)茎直径QTL簇cqSD.A2及其连锁的3个分子标记(Bn-A02-p7893901、Bn-A02-p10176749、Bn-A02-p10668400),QTL cqSD.C8-1及其连锁的2个分子标记(Bn-scaff_25981_1-p90999、Bn-scaff_16287_1-p366585)可用于分子标记辅助育种。本研究进一步丰富了甘蓝型油菜抗茎倒的遗传机理,为茎直径性状精细定位及分子标记辅助育种奠定了基础。

    Abstract:

    Lodging not only reduces the yield, but also is the most important factor affecting mechanized harvesting. Improving the strength of stalk is an important target for lodging-resistant breeding.In this study, a doubled haploid (DH) population (GZ-DH population) developed via microspore culture from a cross F1 between the G922 and Zhongshuang 11 (ZS11) was used as material, quantitative trait loci (QTL) mapping for stalk strength and its related traits were performed. The main results are as follows: (1) The F1 progeny crossed by ZS11 and G922 had significant mid-parent heterosis in three traits, stalk diameter (SD), stalk rind thickness (SRT) and stalk bending strength (SBS). The correlation between the SBS and four traits, SD, SRT, stalk xylem thickness (SXT), rind penetrometer resistance (RPR), as well as between the RPR and SXT, were all positively significant correlation. (2) A genetic linkage map was constructed with 1984 SNP markers, and the total length was 2592.64 cM. 90 QTLs for stalk strength and its related traits were detected in four environments, in which 17 QTLs were identified in more than two environments, including 6 QTLs for SD, 5 QTLs for stalk strength (SS), 2 QTLs for SRT and 4 QTLs for SBS. The major QTL of SD, cqSD.C8-1 could be detected repeatedly in all four environments, and explained 14.67% of phenotypic variation. Two QTL clusters were found on chromosome A2 and C6, respectively, cqSD.A2 consisting of four SD QTLs and cqSS.C6 consisting of four SS QTLs. (3) Stem diameter QTL cluster cqSD.A2 and its three linked molecular markers (Bn-A02-p7893901, Bn-A02-p10176749, Bn-A02-p10668400), QTL cqSD.C8-1 and its two linked molecular markers (Bn scaff_25981_1-p90999, Bn scaff_16287_1-p366585) can be used for molecular marker assisted breeding. This study further enriches the genetic mechanism of stem lodging resistance in Brassica napus, laying the foundation for fine mapping of stem diameter traits and molecular marker assisted breeding.

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徐亮,李开祥,郭少敏,等.甘蓝型油菜茎秆强度相关性状QTL分析[J].植物遗传资源学报,2024,25(12):2031-2041.

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  • 收稿日期:2024-03-01
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  • 在线发布日期: 2024-12-06
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