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首页 > 过刊浏览>2025年第26卷第4期 >817-829. DOI:10.13430/j.cnki.jpgr.20240705002
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‘中油蟠9号’桃套袋果实花色苷合成的转录组-代谢组联合分析
作者:
作者单位:

1.河南科技学院园艺园林学院/河南省特色园艺植物开发利用工程技术研究中心/新乡市果树种质资源与 遗传育种重点实验室，新乡453003;2.中国农业科学院郑州果树研究所，郑州 450000

作者简介:

研究方向为桃果实色泽形成机理解析，E-mail：dingtiyu@hist.edu.cn

通讯作者:

赵亚林，研究方向为桃芽休眠和果实色泽形成机理解析，E-mail：zhaoyalin@hist.edu.cn

基金项目:

河南省科技攻关项目（222102110440，232102111083，232102111087）


Combined Transcriptome-Metabolome Analysis of Anthocyanin Synthesis in ‘Zhongyoupan 9’ Peach Fruits under Bagging Condition
Author:
  • DING Tiyu 1

    DING Tiyu

    School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003
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  • MA Xinxin 1

    MA Xinxin

    School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003
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  • YU Xueli 1

    YU Xueli

    School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003
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  • ZHOU Ruijin 1

    ZHOU Ruijin

    School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003
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  • WANG Lirong 2

    WANG Lirong

    Zhengzhou Fruit Research Institute， Chinese Academy of Agricultural Sciences， Zhengzhou 450000
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  • ZHAO Yalin 1

    ZHAO Yalin

    School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003
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Affiliation:

1.School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003;2.Zhengzhou Fruit Research Institute， Chinese Academy of Agricultural Sciences， Zhengzhou 450000

Fund Project:

Foundation project： Science and Technology Program of Henan Province （222102110440，232102111083， 232102111087）

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    摘要:

    油蟠桃‘中油蟠9号’果实套袋后外果皮有花色苷合成呈现色泽微红，此类型桃适宜套袋栽培，满足消费者对色泽品相的追求。本研究以‘中油蟠9号’盛花后89 d和102 d套袋果实外果皮为试验材料，对‘中油蟠9号’果实套袋后色泽形成原因进行转录组、代谢组以及双组学联合分析。结果表明，转录组分析共鉴定2652个显著差异表达基因，其中与花色苷合成相关的差异表达基因有16个，如花色苷合成途径早期结构基因PpPAL，PpC4H，Pp4CLs，PpCHSs，PtpCHIs，PpF3H，PpF3′H，花色苷合成途径晚期结构基因PpDFR，PpANS，PpUFGT，PpGST，以及56个特异性上调表达的转录因子和光受体基因PpCRY3和PpUVR8.3，这些基因均上调表达，表达模式与花色苷含量相关。利用高效液相串联质谱法检测发育阶段外果皮中类黄酮代谢物的相对含量变化，共检测到112种显著差异代谢物，其中矢车菊素-3，5-二-O-葡萄糖苷显著上调，Log2FC差异倍数最高为16.8。矢车菊素-3，5-O-葡萄糖苷是‘中油蟠9号’套袋果实外果皮呈现红色的主要代谢物。结合双组学联合分析和转录因子结果，推测套袋遮光下PpBL、PpNAC1上调表达激活花色苷调节基因PpMYB10.1的表达，促进花色苷结构基因催化合成矢车菊素-3，5-二-O-葡萄糖苷，使‘中油蟠9号’外果皮呈现红色。研究结果有助于分析遮光下桃果实花色苷合成模式，为筛选更多适宜套袋栽培的优质蟠桃品种提供理论依据。

    Abstract:

    Bagging-produced flat peaches have gained increasing consumer preference due to their reduced fruit cracking， enhanced visual appeal， and golden to slightly reddish fruit surface. The cultivar ‘Zhongyoupan 9’ shows a distinctive reddish coloration under bagging conditions， attributed to anthocyanin synthesis. This characteristic makes it particularly suitable for bagging cultivation， aligning with consumer preferences for optimal color and appearance. In this study， we investigated the exocarp of bagged fruits of 'Zhongyoupan 9' at 89 and 102 days after blooming （DAFB）， through an integrated approach combining transcriptomic， metabolomic， and bi-histological analyses to elucidate the coloration mechanism. Transcriptome analysis identified 2652 significant differential genes， including 16 genes associated with anthocyanin biosynthesis （PpPAL， PpC4H， Pp4CLs， PpCHSs， PpCHIs， PpF3H， PpF3'H， PpDFR， PpANS， PpUFGT， PpGST）， 56 specifically up-regulated transcription factors， and phytoreceptors PpCRY3， PpUVR8.3. These genes were up-regulated and expressed correlating with anthocyanin content. Metabolomic profiling using high performance liquid tandem mass spectrometry detected 112 significantly differential metabolites， with cyanidin-3，5-O-diglucoside showing the most substantial up-regulation （Log2FC = 16.8）. This metabolite was identified as the primary contributor to the red coloration in the exocarp after bagging. Integrative analysis of transcriptomic and metabolomics data， combined with transcription factor profiling， revealed that PpBL and PpNAC1 up-regulated and activated the anthocyanin regulatory gene PpMYB10.1. This activation promoted the catalytic synthesis of cyanidin-3，5-O-diglucoside by anthocyanin structural genes under bagging conditions， ultimately resulting in the characteristic red coloration of ‘Zhongyoupan 9’. These findings advance our understanding of anthocyanin accumulation patterns in bagged peach fruits and provide a theoretical basis for selecting optimal peach varieties for bagging cultivation practices.

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丁体玉,马欣欣,于雪丽,等.‘中油蟠9号’桃套袋果实花色苷合成的转录组-代谢组联合分析[J].植物遗传资源学报,2025,26(4):817-829.

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  • 收稿日期:2024-07-05
  • 在线发布日期: 2025-04-03
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