AFLP markers were applied to 30 melon accessions to study polymorphism and cluster analysis.Twenty-five primer combinations were used to generate 262 polymorphic bands.The cluster analysis showed that the accessions within the same mutation or the same origin were assembled in the same group.The result of cluster analysis was basically consistent with the classification according to the ecotype and geographycal origin,which indicates that AFLP markers are useful for genetic analysis of melon accessions.