A study on inheritance of resistance to bacterial speck disease was carried out by conventional crossing between two resistant and three susceptible varieties of tomato. Resistance in F1, F2 or BC1 was identified by inoculations with bacterial of PST. All the Ft progeny is resistant to the disease. The resistance of F2 progeny was statistically consistent with the ratio of 3 to 1, and the BC1 progeny was accord with the ratio of 1 to 1. The results showed that a single dominant gene controlled the resistance in the two tomato varieties, Hong Zhen Zhu and Mei Wei Ying Tao.