In this study, 57 resistant soybean collections from both China and US were used as materials. They were detect for SNPs at rhg 1 and Rhg 4 loci by using single based chain extension with a microsphere-based assay. The purpose was to illustrate the distribution of alleles at SNPs loci in Chinese accessions and provides the information for utilization of SCN resistant accessions in China. The comprehensive resistances of Chinese soybean SCN resistant accessions were more related to the SNPs genotypes compared to resistance for each SCN race. At both rhg 1 and Rhg 4 loci, 7 of 9 US resistant accessions appeared to be resistant genotypes, and 37 of 49 had resistant genotypes too, taking 77 8% and 66 7% of the total accessions, respectively. Based on this result, it was postulated that most of the Chinese resistant accessions in this study had resistance by interaction of both rhg 1 and Rhg 4, but the other resistant accessions may have different resistant mechanism.