The genetic diversity of rubber tree was analyzed by using RAPD. There were 18 primers showing abundant polymorphisms. The 18 primers produced 128 polymorphic bands. The results of cluster analysis based on Nei Li coefficient revealed that these 20 samples were divided into two main groups. Five wild germplasm were clustered into wild germplasm group, and 12 cultivated germplasm and three wild germpladm were clustered into cultivated germplasm group. The results showed that RAPD marker was suitable for study on genetic diversity in rubber tree, and offering the molecular basis, on which the genetic substance of wild rubber tree was transferred to cultivated rubber tree.