Through the analysis of genetic diversity for 48 taro materials of Yunnan province using five isozymes of esterase, peroxidase, superoxidase, polyphenol oxidase, cytochrome oxidase and cluster analysis for their bands determined, the results showed that band number of five isozymes was between 11 and 19, the number of zymograms was all more than half number of materials determined except that SOD only showed three zymograms. Compared with their morphological characters, cluster result could be explained rationally by the source, use, zoological location, petiole color or the color of leaf center and preliminary classification could be made. Therefore, taro germplasm resources of Yunnan province presented great genetic diversity through observation of morphological characters, existence of wild, semi-wild varieties and isozyme analysis at protein expression level. The results could be used for further researches of taro genetic variation and in situ preservation for taro genetic resources in Yunnan province, which are facing great threat now.