Forty-eight sorghum accessions were genotyped with 104 SSR markers using the TP-M13 automated fluorescent-labelled system. In the method, one reverse SSR primer was tailed with a M13 forward primer, which is called TP-M13, the second SSR primer was normal, and the third primer was the M13 forward primer labeled by FAM. The PCR fragments amplified by these three primers were detected using an automated fluorescent-labelled system (DNA sequencer such as ABI3700). The result indicated that the method had the advantages of high-throughput, sensitiveness, cost-effectiveness and high accuracy over some of other conventional methods. Therefore, the TP-M13 automated fluorescent detection system is suggested to be suitable for genotyping limited number of accessions by the use of a number of SSR markers.