Evaluation of allelopathic effect in rice germplasm is the basis of breeding for rice varieties with allelopathic characteristic, which can inhibit the growth of weeds, reduce the use of chemicals, and promote the improvement of environment. In this paper, the main progress of evaluation methods for allelopathy in rice germplasm is summarized. Achievements of the physiologicad, biochemical and genetic mechanism of allelopathy in rice germplasm are briefly reviewed. Some suggestions on research and utilization of allelopathic rice germplasm have been put forward.