Phytophthora root rot caused by Phytophthora sojae is a destructive disease for soybeans throughout the soybean production regions of world. Utilization of resistant varieties is the most economical and environmentally safe method for controlling the disease. The study screened for resistance of wild soybeans to Phytophthora root rot and investigated their geographical distribution. Four hundred and twelve wild soybean accessions were identified for their reaction to P. sojae strain USAR1 with virulence 1a, 1d,2,3b,5,7 using an agar pluginoculation in seedling. 13.4 % accessions were resistant and 15.3 % accessions were intermediate response. The results indicated that the distribution of Phytophthora resistant wild soybeans was extensive in China, in which it was the most abundant in Anhui province.