Carotenoids is one of the most important member of plant pigments and has diverse functions such as antioxidant,preventing cardiovascular disease and cancer,antiaging.It is a new trend to breed rapeseed cultivar with high carotenoid(HC) content,since HC canola oil may enhance nutrition value and stability.Carotenoids was extracted from rapeseeds in this experiment,and the influence of different solvents,time,the ration of rapeseeds on extraction process was studied,and the optimum condition was obtained.We set up a fast,accurate,simple and convienient method to measure the total carotenoids content in rapeseeds,and the contents of 30 accession of rapeseed germplasm were determined by using this method.The established method provide a good base for the exploiting of high carotenoid rape germplasm and quality breeding for the high carotenoid rape.