A lot studies in wheat molecular biology need to do PCR testing to many accessions of wheat samples. Thus it is very necessary to establish a rapid DNA isolation in wheat. A rapid DNA isolation method built in rice and maize was tried in wheat after performing some modifications. The modified DNA extraction method needs even less time. With the DNA isolated with this method, 1.5kb fragment could be amplified stably. Besides the DNA quantity extracted with this method was quite considerable, one sample could meet 50-100 PCR reactions. So the DNA samples from this rapid isolation method can also be used to do primer selection, gene mapping or other PCR assays.