According to the principle that geographical and genetical distant materials have high heterosis because of genes complement, we crossed high yield and good agronomic characters line origining from Japanese soybean blood as female parent and a promising line having high protein content and derived from wild soybean as male parent. The soybean frogeye leaf spot was inoculated in early generations, and in advanced generations the quality had been analyzed and the soybean phytothora rot was inoculated. By directed selective breeding the new elite soybean germplasm that have multiple high protein, diseases resistance and high yield genes was derived, it had abundant genetic basis, its protein content was above 45 %, and it was resistant to frogeye leaf spot and phytothora rot.