The genetic diversity was evaluated in the main soybean cultivars of Hebei province based on SSR,AFLP and agronomic characters for the utilization and innovation of these germplasm resources.The materials were divided into three groups and two special cultivars in the cluster based on the 12 agronomic traits.The groups could not reflect the pedigrees of the cultivars.Four SAGs(SSR&AFLP-based groups) were generated from the result of SSR and AFLP cluster analysis.Totally 135 SSR alleles were revealed in 41 cultivars.The number of alleles per SSR locus varied from 2 to 8 and with the average of 4.47.The distribution of simpson index of 30 SSR loci was from 0.0928 to 0.7800,and with the mean of 0.6442.Ninety-three DNA polymorphic markers were scored from amplifications of 10 AFLP primer combinations,with 9.3 markers for each primer combination in average.The range of Simple match coefficient was from 0.5877 to 0.9868 and the range of average was from 0.6732 to 0.7653,with the total average of 0.7237.The result indicated that the soybean population was with lower genetic variation.