The genetic relationships among 38 wheat cultivars,which were derived from 1B/1R in the Yellow-Huai River Zone,were investigated by SSR.The results showed that 39 SSR primers produced 186 distinctive bands in total and 143 of them were polymorphic,accounted for 76.9%.Each primer amplified 1 to 9 polymorphic bands with the average of 3.7.The value of allelic polymorphism information content(PIC) ranged from 0.320 to 0.857 on an average of 0.634 per primer.At the level of GD 0.32,cluster analysis showed that 38 wheat lines could be classified into 6 groups.The GD varied from 0.10769 to 0.48571.The results indicated that lower genetic diversity and narrow genetic basis were presented in the 38 wheat cultivars.