The resistance to spread of Fusarium head blight was evaluated in Barley by using single floret injection. The result showed there was the resistance to spread as well as to initial infection of Fusarium head blight in barley. The comparison of different traits in different days after inoculation suggested that the proportion of scabbed spikelets on 21 days after inoculation was the best index for evaluating the resistance to spread of Fusarium head blight, which had the largest range, genetic coefficient variance and heretability. The number of scabbed spikelets on 21 days after inoculation and the area under disease progress curve were significant correlated to the proportion of scabbed spikelets on 21 days after inoculation. There was a large diversity for the resistance to spread of Fusarium head blight in barley cultivar. Among all tested cuhivars, Suyin 21, Wujing 1, Pu846193, Yan 97001 and 96AC20-30 were high resistance cuhivars, which occupyied 2.04% of the total tested cuhivars.