During long time evolution,plants developed elaborate defense pathway and complicated mechanisms against virus and other pathogens. Virus proteins,such as coat protein(CP),movement protein(MP) and polymerase protein,would interact with host plant proteins during virus infection process.The yeast two hybrid system is a useful method to analyze the interaction between proteins in vitro and applied widely in studying the interaction between proteins of plant and virus.This article mainly presented the advances of the protein interaction of plant and virus by using yeast two hybrid during the past decade.