36 accessions of Triticum aestivum ssp.yunnanense King were used to evaluate the HMW glutenin subunits at Glu-1 loci by SDS-PAGE.3 HMW glutenin subunits(i.e.N,2* and 1),5(i.e.7,7 8,17 18,13 16 and 6 8),and 1(2 12) were found at Glu-A1,Glu-B1 and Glu-D1 loci,respectively.Totally there were 6 components of HMW-GS in Yunnan hulled wheat,including N,7,2 12;N,7 8,2 12;2*,7 8,2 12;2*,17 18,2 12;1,6 8,2 12 and 1,13 16,2 12.When compared with the HMW glutenin subunits found in bread wheat,subunits in Yunnan hulled wheat accounted for 18%,and its Nei's genetic variation index of genomes A,B and D was 0.2484,0.5734 and 0,ranking as B>A>D,indicating that Yunnan hulled wheat is an ancestral subspece and there was no genetic diversity at D genome.The average score based on the Payne's system was 5.2,and the highest 3 accessions reached 8.42%.Yunnan hulled wheat had the components of 1,7 8,2 12 and N,7 8,2 12,confirmed as good subunits for steam-bread making quality,which could be used for wheat steam-bread quality improvement.