The Screening of SSR and STS Markers for Genetic Diversity Analysis of Mungbean Germplasm
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S635.1

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    Abstract:

    At present,PCR markers for genetic diversity analysis of mungbean(Vigna radiate) germplasm are much limited.Twelve mungbean accessions with different agronomic traits were used to screen polymorphic PCR markers that were obtained from mungbean,adzuki bean(Vigna angularis),cowpea(Vigna unguiculata) and common bean(Phaseolus vulgaris).The results showed that 35 markers among 41 mungbean SSR primer pairs were effectively amplificated and 6 were polymorphic;22 among 28 mungbean STS primer pairs were effectively amplificated and 2 were polymorphic;6 among 8 adzuki bean SSR primer pairs were effectively amplificated,but no polymorphic was found;17 among 27 cowpea SSR primer pairs were effectively amplificated,one was polymorphic;9 primer pairs had good amplification product among 24 common bean SSR primer pairs,one of them was polymorphic.These polymorphic primers will be useful for genetic diversity analysis of Chinese mungbean germplasm.

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History
  • Received:April 19,2007
  • Revised:July 02,2007
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